Thanks for looking into this. I have unzipped all the .gz files so that the
directory contains all .chain files. The liftover.loc file has also been
modified to refer to all the .chain files instead, as such:
I still run into the same error if I try to run the tool in Galaxy, though
(hg19ToMm9 mapping currently not available). The liftover command still
works fine through the command line.
I have a feeling the error has to do with the issue with Galaxy's job
runner returning anything written to stderr as an error, mentioned here:
Seems like it was an issue back in 2010. Has this been rectified?
Again, thank you for your help,
On Wed, Jul 4, 2012 at 9:42 PM, Jennifer Jackson <j...@bx.psu.edu> wrote:
> Hello Kwon,
> Most of this sounds correct. The only issue is with compressed files.
> Galaxy uses uncompressed liftOver data at our site and this is how we
> instruct that it be set up (see
> Would you be able to run a test to see if uncompressing resolves the
> issue? Uncompress one of the over.chain files, modify the .loc file so that
> the name also reflects the uncompressed naming, then try to use the UI
> version of liftOver with this uncompressed data. Please let us know how
> this works out (cc-ing the list, so that others can learn of the confirmed
> For now, there is no automated way to enter the .loc data entries. There
> is some development activity going on right now to address data setup
> (including liftOver), but nothing is ready yet to be shared. When complete,
> any new tools will be announced (loudly - we know that they are highly
> Galaxy team
> On 7/3/12 10:21 PM, Kwon SoonJae wrote:
> Hi Everyone,
> I installed a local instance of galaxy and some tools using the command
> line, and for some reason liftover will work in the command line but won't
> work in galaxy. Here's what I've done so far:
> - Downloaded liftover standalone executable from the tool-dependencies
> page in the Gwiki.
> - Changed permissions on liftover executable to make it usable in the
> command prompt (terminal, macosx) -> otherwise I get permission denied.
> - Copied executable over to /usr/local/bin likewise to use in command
> prompt and in Galaxy.
> - Modified liftover.loc to include all the conversion types and
> directories of chain.gz files I downloaded to use with the tool (I believe
> liftover can make use of both .chain and .chain.gz files).
> After that I tried to run liftover in Galaxy and it didn't work, giving
> me some error that said hg19ToMm9 mapping is currently not available. It
> seems to work fine using the command line though.
> Since my goal is to setup galaxy locally, I really want to get this
> working correctly. Can anyone guide me through what's gone wrong?
> Also, there must be a better way of installing all these tools than
> manually downloading every single one and using the command line, surely? I
> had to type out the format of liftover.loc file manually for around 130
> chain files... I'm convinced there has to be a better way.
> Thanks in advance,
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> Jennifer Jacksonhttp://galaxyproject.org
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