Hello Kwon,
There are no known issues with the liftOver wrapper (tools / extract /
liftOver_wrapper.py and liftOver_wrapper.xml).
Three more troubleshooting ideas:
1 - This may sound simple, but have you restarted the database since you
updated the .loc files?
http://wiki.g2.bx.psu.edu/Admin/Data%20Integration (final step)
2 - Remove the relative path from the .loc file entries (the "~/")) and
replace with an explicit full path. I personally have not seen this used
before in .loc files. Perhaps others on the list can comment about
whether they have been able to use this successfully and if any special
configuration was necessary.
3- Confirm that the genomes are in your builds list (also covered in
wiki above). Note that UCSC "dbkey" values have special capitalization
rules depending on where they are used. Your example .loc file has this
correct, but I recommend that you also check your builds list entries
against the wiki examples and your actual data. If you make any changes,
be sure to restart before testing.
For example, the liftOver file pair: hg19ToDanRer6.over.chain &
danRer6ToHg19.over.chain
human dbkey = hg19
zebrafish dbkey = danRer6
Please let us know how this works out,
Jen
Galaxy team
On 7/4/12 7:35 PM, Kwon SoonJae wrote:
Hi Jen,
Thanks for looking into this. I have unzipped all the .gz files so
that the directory contains all .chain files. The liftover.loc file
has also been modified to refer to all the .chain files instead, as
such: http://pastebin.com/xSKGvUtG
I still run into the same error if I try to run the tool in Galaxy,
though (hg19ToMm9 mapping currently not available). The liftover
command still works fine through the command line.
I have a feeling the error has to do with the issue with Galaxy's job
runner returning anything written to stderr as an error, mentioned
here: http://wiki.g2.bx.psu.edu/Future/Job%20Failure%20When%20stderr
Seems like it was an issue back in 2010. Has this been rectified?
Again, thank you for your help,
SJ
On Wed, Jul 4, 2012 at 9:42 PM, Jennifer Jackson <[email protected]
<mailto:[email protected]>> wrote:
Hello Kwon,
Most of this sounds correct. The only issue is with compressed
files. Galaxy uses uncompressed liftOver data at our site and this
is how we instruct that it be set up (see
galaxy-central/tool-data/liftOver.loc.sample).
Would you be able to run a test to see if uncompressing resolves
the issue? Uncompress one of the over.chain files, modify the .loc
file so that the name also reflects the uncompressed naming, then
try to use the UI version of liftOver with this uncompressed data.
Please let us know how this works out (cc-ing the list, so that
others can learn of the confirmed dependency).
For now, there is no automated way to enter the .loc data entries.
There is some development activity going on right now to address
data setup (including liftOver), but nothing is ready yet to be
shared. When complete, any new tools will be announced (loudly -
we know that they are highly desired).
Thanks!
Jen
Galaxy team
On 7/3/12 10:21 PM, Kwon SoonJae wrote:
Hi Everyone,
I installed a local instance of galaxy and some tools using the
command line, and for some reason liftover will work in the
command line but won't work in galaxy. Here's what I've done so far:
- Downloaded liftover standalone executable from the
tool-dependencies page in the Gwiki.
- Changed permissions on liftover executable to make it usable in
the command prompt (terminal, macosx) -> otherwise I get
permission denied.
- Copied executable over to /usr/local/bin likewise to use in
command prompt and in Galaxy.
- Modified liftover.loc to include all the conversion types and
directories of chain.gz files I downloaded to use with the tool
(I believe liftover can make use of both .chain and .chain.gz files).
After that I tried to run liftover in Galaxy and it didn't work,
giving me some error that said hg19ToMm9 mapping is currently not
available. It seems to work fine using the command line though.
Since my goal is to setup galaxy locally, I really want to get
this working correctly. Can anyone guide me through what's gone
wrong?
Also, there must be a better way of installing all these tools
than manually downloading every single one and using the command
line, surely? I had to type out the format of liftover.loc file
manually for around 130 chain files... I'm convinced there has to
be a better way.
Thanks in advance,
SJ
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Jennifer Jackson
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Jennifer Jackson
http://galaxyproject.org
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