On Tue, Sep 11, 2012 at 3:11 PM, <kauerb...@comcast.net> wrote: > Hello, > > > > Can any one please tell me what could be causing this error message when I > try to submit a blast job to Galaxy? > > > An error occurred running this job:BLAST Database error: No alias or index > file found for protein database > [/9720/genome_references/ncbi/nr-protein-db/nr-newstyle/nr] in search path > [/9720/galaxyprod/galaxy-dist/database/job_working_directory/1454::] > Return error code 2 from command:blast:blastx.
That means that BLAST+ didn't find the NR database files. What does this give at the command line? ls /9720/genome_references/ncbi/nr-protein-db/nr-newstyle/nr* You should have a lot of files including nr.pal, nr.00.phd, ..,. nr.07.psq Perhaps you've got the wrong BLAST database path setup in the blastdb_p.loc file? Peter ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/