- It needs to be a tab not spaces between the fields in that loc file
- can you confirm that it is a tab? (Many editors will implicitly
replace tabs with spaces.)
- You need to restart Galaxy after updating that file - can you
confirm it has been restarted?

-John

On Fri, Jul 22, 2016 at 11:34 AM, Alexandre Maucuer
<alexandre.mauc...@inserm.fr> wrote:
> Hi,
> I've installed a Galaxy instance on a local machine under ubuntu 16.04.
> I am trying to fetch sequences from hg19.2bit I downloaded from ucsc as
> ~/galaxy/depot/data2/galaxy/hg19$hg19.bit
> I added:
> hg19    /depot/data2/galaxy/hg19/hg19.2bit
> to my twobit.loc file in tool-data directory
>
>
> When running the extract genomic dna, I get the error:
> "No sequences are available for 'hg19', request them by reporting this
> error."
> see also attached screenshot
> Thanks in advance for your help
>
> --
> Alexandre Maucuer, Ph.D.,
> INSERM, France
>
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