I have single end Illumina reads from ChIP-Seq experiment. The files have
encoding Illumina 1.5, and the sequence length is 76bp.
After basic FastQc I want to map the sequences using Bowtie. My question is:
do I need to split my reads (farward and backward) before running mapping tool?
In one of Galaxy screen shorts reads are spitted while not in the other.
Thank you in advance,
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:
To manage your subscriptions to this and other Galaxy lists,
please use the interface at: