Hi Robin,

Run the FASTQ Groomer on your datafile first, then use the result as input to the Compute quality statistics tool. (Skip using Quality format converter).

Hopefully this helps,

Jen
Galaxy team

On 5/3/11 2:00 AM, Robin Mjelle wrote:
Dear User,

I am trying to use the tool "Compute quality statistics
<http://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastx_quality_statistics>"
in galaxy on Ilumina single reads. The file is 2.3 Gb, fastq format. I
have performed Quality format converter on the data set and the format
is now qualillumina. Despite of this, galaxy don't recognize any dataset
in the workflow to use as input into quality statistics.
Any idea why my dataset is not accepted as input?

Best,

Robin



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