For tools that allow a custom genome, the form will include an option to
use a "Dataset from the history" (or similar). Then a sub-menu will pop
up where the reference genome can be selected.
For the query sequences, Fastq or fasta format may be required. After
upload, make certain the datatype is a fastq version and then run "Fastq
Groomer". Most alignment tools require a groomed file.
For the target genome, fasta format is required. After upload, this can
be assigned if needed on the ""Edit Attributes" form (click on pencil icon).
Hopefully this helps,
On 10/6/11 8:57 AM, dtr...@ira.cinvestav.mx wrote:
I WANT TO ALIGNMENT MILLIONS OF SEQUENCES FROM A sRNA LIBRARY WITH A SMALL
GENOME (2600 BASES). HOW CAN I DO THIS ALIGNMENT IN GALAXY?. I HAVE TRIED
WITH MULTIPLE ALIGNMENTS FROM TOOLS, BUT I CAN NOT SELECT BOTH FILES.
THANKS FOR YOUR HELP
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