Hi Dominique,

Glad that helped. And yes, you can merge many file types that are text-based with the tool 'Text Manipulation -> Concatenate datasets". Sometimes you will need to convert to format tabular first, and then back to the desired format (fasta, gtf, etc.) after.


Take care,

Jen
Galaxy team

On 9/19/13 5:51 AM, D. A. Cowart wrote:
Thank you Jennifer, this helped tremendously. I completely missed the barcode splitter too. One other question: do you know if it is possible to merge different fasta files on Galaxy? Say, I wanted to merge those tagged files back to one complete fasta file.

Best,
Dominique Cowart


On Thu, Sep 19, 2013 at 12:19 AM, Jennifer Jackson <j...@bx.psu.edu <mailto:j...@bx.psu.edu>> wrote:

    Hello Dominique,

    Yes, this can be done. Here is the process ->

    Start by splitting up the data by using the 'NGS: QC and
    manipulation -> Barcode Splitter" tool. The result files will be
    available as links. These can be copied and added to the "Get Data
    -> Upload File" tool in the text box, in batch, and each will
    loaded as a dataset. Copying them into a simple text file, then
    pasting into the Upload tool all at once is a quick way to do
    this, or you can do one by one.

    Once you have the individual files as datasets, you probably will
    want to rename them to better keep track of which barcode/tag they
    represent. Click on the pencil icon in the upper right corner of
    each dataset to do this on the Edit Attributes form.

    Next, the idea is to convert the fasta dataset to tabular, add in
    a column with the "_Tag1" information, merge the original
    identifier column with the new tag column, cut the columns to
    rearrange - (you want just the new merged identifier and the
    original fasta sequence - leaving behind the two columns with the
    original identifier + tag), then covert back from tabular to fasta
    format. Use the tools in 'Text Manipulation' and 'FASTA
    manipulation' to do these operations. I would normally suggest
    creating/using a workflow at this point, but as the tags will all
    be different, and the "Add column" step is in the middle of the
    processing, this is probably not worth it.

    Hopefully this helps!

    Jen
    Galaxy team


    On 9/18/13 7:36 AM, D. A. Cowart wrote:
    Hello,

    I need to perform an action (or series of actions) on an 454
    dataset using Galaxy, and have not been able to figure out the
    necessary steps, even after looking through the toolbar
    expressions and using custom search.
    My file is a fasta and has the standard format:

    >GNJQDEZ01A940A
    CTGAGTCAGGTCAACAATCATAAGATATTGGCACCATGTACCTGTGGTTCTCGTTTCC
    ATGTTA
    >GNJQDEZ01BJYQZ
    CTGAGTCAGGTCAACAATCATAAGACATCGGCTCTCTATATTTAATATTGGT

    Each of the 100,000 sequences within this file contains a
    specific tag, which is the first 8 nucleotides.
    There are 19 tags total. I would like to identify these tags and
    add an identifier of the tag to the sequence name.
    Therefore, if I am looking for the first tag (CTGAGTCA), the
    output would look like:

    >GNJQDEZ01A940A_*Tag1*
    *CTGAGTCA*GGTCAACAATCATAAGATATTGGCACCATGTACCTGTGGTTCTCGTTTCC
    ATGTTA

    Is it possible to achieve this using Galaxy? If possible, could
    you kindly suggest tools to use.

    Thank you in advance,
    Dominique Cowart


    ___________________________________________________________
    The Galaxy User list should be used for the discussion of
    Galaxy analysis and other features on the public server
    atusegalaxy.org  <http://usegalaxy.org>.  Please keep all replies on the 
list by
    using "reply all" in your mail client.  For discussion of
    local Galaxy instances and the Galaxy source code, please
    use the Galaxy Development list:

       http://lists.bx.psu.edu/listinfo/galaxy-dev

    To manage your subscriptions to this and other Galaxy lists,
    please use the interface at:

       http://lists.bx.psu.edu/

    To search Galaxy mailing lists use the unified search at:

       http://galaxyproject.org/search/mailinglists/

-- Jennifer Hillman-Jackson
    http://galaxyproject.org



--
Jennifer Hillman-Jackson
http://galaxyproject.org

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:

  http://galaxyproject.org/search/mailinglists/

Reply via email to