I installed BLAT on a local server and successfully mapped Agilent's 1M array probes.
>X-Original-To: [email protected] >Delivered-To: [email protected] >X-IronPort-Anti-Spam-Filtered: true >X-IronPort-Anti-Spam-Result: AswEAAtS8UqAcjBtnGdsb2JhbABMgQOaDgEBAQEBCAsICRMus1aGFYhMhD0EhQiGZg >X-IronPort-AV: E=Sophos;i="4.44,681,1249272000"; d="scan'208";a="283081488" >X-SBRS: 5.6 >X-ASG-Debug-ID: 1257346540-365300d00000-3DImsM >X-Barracuda-URL: http://mailgw.cse.ucsc.edu:8000/cgi-bin/mark.cgi >X-Barracuda-Envelope-From: [email protected] >X-IronPort-AV: E=Sophos;i="4.44,680,1249272000"; d="scan'208";a="296557665" >X-Barracuda-BBL-IP: 10.181.25.43 >X-Barracuda-RBL-IP: 10.181.25.43 >From: Hariharan Easwaran <[email protected]> >To: "[email protected]" <[email protected]> >Date: Wed, 4 Nov 2009 09:55:39 -0500 >X-ASG-Orig-Subj: Help with BLAT >Thread-Topic: Help with BLAT >Thread-Index: AQHKXV7f2dBik57GLUKW4vkeFkEmbg== >Accept-Language: en-US >Content-Language: en-US >X-MS-Has-Attach: >X-MS-TNEF-Correlator: >acceptlanguage: en-US >MIME-Version: 1.0 >X-Barracuda-Connect: ipex4.johnshopkins.edu[128.220.161.141] >X-Barracuda-Start-Time: 1257346541 >X-Barracuda-Encrypted: RC4-SHA >X-Barracuda-Virus-Scanned: by Barracuda Spam & Virus Firewall at soe.ucsc.edu >X-Barracuda-Spam-Score: 0.00 >X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=5.0 KILL_LEVEL=9.0 tests= >X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.13712 Rule breakdown below pts rule name description ---- ---------------------- -------------------------------------------------- >X-MIME-Autoconverted: from quoted-printable to 8bit by lists.soe.ucsc.edu id nA4Etkx7029470 >X-Mailman-Approved-At: Wed, 04 Nov 2009 10:11:44 -0800 >Subject: [Genome] Help with BLAT >X-BeenThere: [email protected] >X-Mailman-Version: 2.1.9 >List-Id: UCSC Genome Browser discussion list <genome.lists.soe.ucsc.edu> >List-Unsubscribe: <https://lists.soe.ucsc.edu/mailman/listinfo/genome>, <mailto:[email protected]?subject=unsubscribe> >List-Archive: <http://lists.soe.ucsc.edu/pipermail/genome> >List-Post: <mailto:[email protected]> >List-Help: <mailto:[email protected]?subject=help> >List-Subscribe: <https://lists.soe.ucsc.edu/mailman/listinfo/genome>, <mailto:[email protected]?subject=subscribe> >Content-Transfer-Encoding: 7bit > >Dear Madam/Sir, >I would like to BLAT a whole probe set (about 44,000 probes, Agilent) to map >it to the human genome and get the chromosomal coordinates and corresponding genes. >However, the UCSC browser allows uploading only 25 sequences at a time. Is there a way to upload 44000 sequences? > >I would really appreciate you help. > >Sincerely, >Hari Easwaran >Johns Hopkins University >Baltimore, MD 21231 >_______________________________________________ >Genome maillist - [email protected] >https://lists.soe.ucsc.edu/mailman/listinfo/genome Jianhua Zhang Department of Medical Oncology Dana-Farber Cancer Institute 44 Binney Street Boston, MA 02115-6084 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
