Dear Professor, According your previous reply, "This is why you see overlap between the 5’ UTR of uc010nxq.1 and the 3’ UTR of uc001aaa.3 and this is perfectly fine." In biology behaviour of human genome, is it normal that 3'-UTR overlap with 5'-UTR? Can I know that whether UCSC got provide the coordinates of human miRNA? If yes, can I know how to extract it out? I got try to extract out the human exon coordinates by using the following method: 1. Set the following options: Group: Genes and Gene Prediction Tracks Track: RefSeq Genes Table: knownGene Output format: sequence
2. Click the "get output" button 3. Select "genomic" and click the "submit" button 4. Check the " CDS Exons " 5. Click the "get sequence" button From the result, it shown that >hg19_refGene_NM_032291 range=chr1:67000042-67208778 5'pad=0 3'pad=0 strand=+ >repeatMasking=none However, when I check the coordinates through UCSC Genome Browser, it shown that chr1:67,000,042-67,208,778 is fall in Gene region but not exons of that particular gene. Can I know how to just extract the coordinate of each exon in every gene? My main purpose just hope can extract the coordinates of promoter, 3'-UTR, 5'-UTR, miRNA, rRNA, tRNA, exon position of human by using UCSC. Really thanks for your advice. Many thanks for your explanation in detail previously. Very helpful and learn a lot from your explanation. I'm very fresh at bioinformatics. Thanks. best regards Edge Research Student _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
