It is best to remove the blocks and let them set at room temperature. Leaving them at 60 or more degrees can adversely affect antigens.
We routinely do this with our autopsies and have not noted any issues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: P Sicurello via Histonet [mailto:[email protected]] Sent: Wednesday, 17 May 2017 1:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
