I agree with Tony. I have on many occasions left the block baskets sit at room temperature (sat them in an OFF oven) and then turned the oven on to temp and re-embedded the tissue with no issues. You could also just return the basket to the warming tray until they are warm again to embed.
Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor ________________________________________ From: [email protected] [[email protected]] Sent: Thursday, May 18, 2017 1:00 PM To: [email protected] Subject: Histonet Digest, Vol 162, Issue 17 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histotech and Histology Supervisor job openings in Birmingham, AL (Brannon Owens) 2. Re: A Question About Paraffin Times (Tony Henwood (SCHN)) 3. Re: A Question About Paraffin Times (Garrey Faller) 4. Decal Solution (Diana Martinez-Longoria) 5. Histo job opportunity in a GI lab-Oak Lawn Illionios (dusko trajkovic) 6. Grosser Needed in Southern California (Paula) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 May 2017 19:40:39 +0000 From: Brannon Owens <[email protected]> To: "[email protected]" <[email protected]> Subject: [Histonet] Histotech and Histology Supervisor job openings in Birmingham, AL Message-ID: <d5421f6f.d4340%[email protected]> Content-Type: text/plain; charset="us-ascii" We are currently seeking candidates for several openings in the Birmingham, AL area. Relocation assistance is offered! Position 1: Histology Supervisor (3rd shift) Position 2: Histotechnician (1st shift) Position 3: Histotechnician (3rd shift) Full job descriptions available on the Allied Search Partners website as well as additional opportunities in the field of histology. We look forward to hearing from you! Thank you, To view a complete list of Allied Search Partners current openings go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com_careers_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=OYAKUwWjo2B60ZnbVZNPcYLy-11xcbih2bPn7znIoII&e= Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.linkedin.com_in_jbrannonowens_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=zbxgUl7AXC4yU7kuDZDyb5271_RwiU2nRuQQCs_vNkQ&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=1k7ul6M58-fTSS7jKYGsyX96kDavLFMZIplAdFFSxmw&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=i1mcP4-2CVuT7joH3hQecsKTINIRN3aC0TUbd9MwfRw&e= > T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 ------------------------------ Message: 2 Date: Wed, 17 May 2017 23:26:24 +0000 From: "Tony Henwood (SCHN)" <[email protected]> To: P Sicurello <[email protected]> Cc: "[email protected]" <[email protected]> Subject: Re: [Histonet] A Question About Paraffin Times Message-ID: <0237449de79dbc45b686ab82cdcd16ff95554...@svdcmbx-mex008.nswhealth.net> Content-Type: text/plain; charset="us-ascii" It is best to remove the blocks and let them set at room temperature. Leaving them at 60 or more degrees can adversely affect antigens. We routinely do this with our autopsies and have not noted any issues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: P Sicurello via Histonet [mailto:[email protected]] Sent: Wednesday, 17 May 2017 1:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 3 Date: Thu, 18 May 2017 07:23:50 -0400 From: Garrey Faller <[email protected]> To: Cindy Bird <[email protected]> Cc: "Logan, Shannon" <[email protected]>, "[email protected]" <[email protected]> Subject: Re: [Histonet] A Question About Paraffin Times Message-ID: <[email protected]> Content-Type: text/plain; charset=us-ascii I have had to deal with this in the past and could not find an answer . My intuition was that it can't be good to leave in hot wax. So, on the rare occasion when my only Histotech called in sick I had to take the blocks out and cool them down. When ready they were put back in the tissue embedder to heat up and everything was fine. That's my experience, Garrey Sent from my iPhone > On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet > <[email protected]> wrote: > > I agree? > > Sent from my iPhone > >> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet >> <[email protected]> wrote: >> >> Hello Paula, >> So why must the cassettes be removed on Saturday morning if you aren't >> embedding until Monday morning? >> Don't you have a "delay start" feature on your processor? Neither option A >> or B seem like a good thing for the tissue! >> We time our processor to finish at 5 AM Monday when the first Histotech >> arrives for embedding. >> The cassettes remain in formalin until the processor starts up on Sunday >> evening. >> >> Regards, >> >> >> Shannon H. Logan B.S., HTL (ASCP) >> Pathology Department >> >> Bellin Health Memorial Hospital >> 744 South Webster Avenue >> Green Bay, WI 54305-3400 >> 920-433-3653 X3727 >> >> >> >> From: P Sicurello via Histonet [mailto:[email protected]] >> Sent: Tuesday, May 16, 2017 10:34 AM >> To: HistoNet >> Subject: [Histonet] A Question About Paraffin Times >> >> Good Morning Listers, >> >> I am asking the collective wisdom of the Histonet this question: >> >> >> Is it better to remove baskets from the processor on Saturday morning and: >> >> A. Let the cassettes freeze, then melt them down and embed Monday morning? >> OR >> B. Leave the cassettes in molten paraffin and embed Monday morning? >> >> >> I am of the opinion that leaving the samples (not fatty, like breast cores) >> in molten paraffin (62 degrees C) is bad practice, and causes them to get >> "crunchy", among other things. >> >> What do you think? >> >> Thank in advance. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 <#> >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail is >> intended only for the person or entity to which it is addressed and may >> contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action in >> reliance upon this information by persons or entities other than the >> intended recipient is prohibited. If you received this e-mail in error, >> please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> [email protected]<mailto:[email protected]> >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= >> >> <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= >> > >> _______________________________________________ >> Histonet mailing list >> [email protected] >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= > > _______________________________________________ > Histonet mailing list > [email protected] > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= ------------------------------ Message: 4 Date: Thu, 18 May 2017 12:50:04 +0000 From: Diana Martinez-Longoria <https://urldefense.proofpoint.com/v2/url?u=http-3A__Diana.Martinez-2DLongoria-40ecrmc.org&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=ArCXb_8wVna_PBL5j1Jrb8I3sMUx6Sq4sa6dGId0DJ4&e= > To: 'Histonet' <[email protected]> Subject: [Histonet] Decal Solution Message-ID: <12b71261212be94bb9fb7735484b9fa101014b8...@exmbx01.ecrmc.ci.el-centro.ca.us> Content-Type: text/plain; charset="iso-8859-1" Good morning everyone, I was wondering if someone can give me advise regarding the best decal solution for bone marrow cores? Our pathologist wants to use a decal solution that has EDTA based for molecular studies (ex: suspected tumor/lymphoma etc. diagnosis). Any advice would be greatly appreciated. Thank you! Diana Martinez-Longoria Bachelors of Science in Biology Histotechnician (ASCP)cm El Centro Regional Medical Center Phone: 760-339-7267 Fax: 760-339-4570 Email:[email protected] ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Message: 5 Date: Thu, 18 May 2017 14:25:21 +0000 (UTC) From: dusko trajkovic <[email protected]> To: "[email protected]" <[email protected]> Subject: [Histonet] Histo job opportunity in a GI lab-Oak Lawn Illionios Message-ID: <[email protected]> Content-Type: text/plain; charset=UTF-8 If anyone is interested in a histology position in a GI lab, please let me know as soon as possible?Lab is located in Oak Lawn https://urldefense.proofpoint.com/v2/url?u=http-3A__Illinois.At&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=thbNW8AAFpx_oiEuo4V1LLh3gMir1ia2MinJ-3muB60&e= the moment I do not have details, but will be getting them soon.ThanksDusko Trajkovic760-390-3478 ------------------------------ Message: 6 Date: Thu, 18 May 2017 09:54:56 -0700 From: "Paula" <[email protected]> To: <[email protected]> Subject: [Histonet] Grosser Needed in Southern California Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello, We need a true PA (Pathologist Assistant) or a highly qualified Grossing person that can do hospital work. Full time or Part Time options. If interested, please contact Jason Cochran (Administrator) for more information. Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330 Thank you, Paula ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= ------------------------------ End of Histonet Digest, Vol 162, Issue 17 *****************************************The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. 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