Thanks for the tip! On Fri, May 1, 2020 at 8:53 AM Val Tolley <vtolle...@gmail.com> wrote:
> It’s difficult to know without your full protocol, but you can easily test > your DAB. > > Take a paper towel and add a drop of your secondary/HRP. Add a drop of > DAB to that. If it doesn’t immediately turn brown, your hydrogen peroxide > has likely degraded into water. Make a fresh batch of DAB and try again. > > > > > On May 1, 2020, at 8:28 AM, Kristy Castillo via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > Hi Histonetters, > > > > We are starting our IHC (fun times), we are having trouble with the DAB > > lighting up. Processed for 5 thru 10 minutes and still nothing. Our > > permanent red is working just fine. Any ideas. > > > > Thank you! > > > > Kristy > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
