Since you are newbies, I am just putting IHC 101 out there.
Permanent red is for alkaline phosphatase detection and DAB is for HRP.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
A sharp knife is nothing without a sharp eye. - Klingon Proverb
On Friday, May 1, 2020, 10:29:00 AM CDT, Kristy Castillo via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB
lighting up. Processed for 5 thru 10 minutes and still nothing. Our
permanent red is working just fine. Any ideas.
Thank you!
Kristy
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