Kristy: I’m thinking along the same lines as Paula; is it possible that your detection reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme? If so, a peroxide/DAB solution simply won’t react with it. Can’t wait to see your protocol, with detailed descriptions of the antigen, pretreatment reagent and ‘labeling’ enzyme. Cheers, Joe Myers, M.S.,CT/QIHC(ASCP)
________________________________ From: Kristy Castillo via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, May 1, 2020 11:00 AM To: histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
