Dear all,
I'd like to use ImageJ-Ops to create an image with "virtual beads" of a
given diameter with a given intensity.
I thought of using a 3D Gaussian to get the spatial intensities for each
bead (at a random subpixel position), and then summing up single bead
images to get a volume with randomly distributed spots.
Finally, I might want to convolve these ideal 3D Gaussian spots with a
given point-spread function (PSF).
I guess I'll need
ij.op().image().equation()
ij.op().math().add()
and finally
ij.op().filter().convolve()
but I am unsure how to proceed.
Could anybody give me some hints how to start?
Thanks,
Jan
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