Hi Brian, thanks a lot for the example script, that's a great starting point!
Cheers, Jan On 19.08.2015 15:52, Brian Northan wrote:
Hi Jan I am not sure of the best to draw points that are at a sub-resolution position. Below is an example that shows how to use imglib2 (in a script) to draw points and then convolve them. (note it works with imagej-ops-0.16.0 and above) https://github.com/imagej/imagej-tutorials/blob/scripting/jython/CreateAndConvolvePoints.py If you know the equation you want to use for a point at sub-resolution maybe you can implement it using the same imglib2 interfaces... On Wed, Aug 19, 2015 at 7:32 AM, Jan Eglinger <jan.eglin...@gmail.com <mailto:jan.eglin...@gmail.com>> wrote: Dear all, I'd like to use ImageJ-Ops to create an image with "virtual beads" of a given diameter with a given intensity. I thought of using a 3D Gaussian to get the spatial intensities for each bead (at a random subpixel position), and then summing up single bead images to get a volume with randomly distributed spots. Finally, I might want to convolve these ideal 3D Gaussian spots with a given point-spread function (PSF). I guess I'll need ij.op().image().equation() ij.op().math().add() and finally ij.op().filter().convolve() but I am unsure how to proceed. Could anybody give me some hints how to start? Thanks, Jan
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