Hi Jan I am not sure of the best to draw points that are at a sub-resolution position.
Below is an example that shows how to use imglib2 (in a script) to draw points and then convolve them. (note it works with imagej-ops-0.16.0 and above) https://github.com/imagej/imagej-tutorials/blob/scripting/jython/CreateAndConvolvePoints.py If you know the equation you want to use for a point at sub-resolution maybe you can implement it using the same imglib2 interfaces... On Wed, Aug 19, 2015 at 7:32 AM, Jan Eglinger <jan.eglin...@gmail.com> wrote: > Dear all, > > I'd like to use ImageJ-Ops to create an image with "virtual beads" of a > given diameter with a given intensity. > I thought of using a 3D Gaussian to get the spatial intensities for each > bead (at a random subpixel position), and then summing up single bead > images to get a volume with randomly distributed spots. > Finally, I might want to convolve these ideal 3D Gaussian spots with a > given point-spread function (PSF). > > I guess I'll need > ij.op().image().equation() > ij.op().math().add() > and finally > ij.op().filter().convolve() > > but I am unsure how to proceed. > > Could anybody give me some hints how to start? > > Thanks, > Jan > > _______________________________________________ > ImageJ-devel mailing list > ImageJ-devel@imagej.net > http://imagej.net/mailman/listinfo/imagej-devel >
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