Thanks Pankaj
I had read it ( a few repeatedly) in some reviews or papers  about the
molecular  exams from old specimen

I have also seen ( i was present when at .....  herbarium)   some visiting
scientists were snipping small/minute leaf  pieces from herbarium dried
leaf etc for exams )

could not have been for enzyme essays!

usha di

ps silica gel is how i dry my flowers for flower arranging for fall and
winter.

would drying in microwave oven preserve the dna/r na structure?/ i have not
thought about it..
is there any data out there?


On Wed, Nov 4, 2015 at 4:06 AM, Pankaj Kumar <[email protected]> wrote:

> Plant specimens were not taken for dna analysis earlier. But now a days
> they are.
> The main thing about DNA analysis is, it is best done from fresh samples
> or a sample which were dried without rotting (very fast drying). Hence we
> use silica gel for fast drying. As the samples on herbarium sheet or even
> in the silica gets older the chances of DNA extraction becomes less as the
> dna starts degenrating.
> Poison can be anything from mercury chloride or even alcohol. Infact at
> our organisation we dont use any chemicals to kill.but still we are not
> able to extract DNA !!
>
> On Tue, Nov 3, 2015 at 10:39 PM, Ushadi Micromini <
> [email protected]> wrote:
>
>> Tapasda
>>
>> poisoned by what ?
>>
>> mercury  or something else?
>>
>> i thought some pieces of leaves are routinely taken for biochemical and
>> molecular analysis from herbarium specimen
>>
>> am i wrong?
>>
>> usha di
>>
>>
>>
>>
>> On Mon, Nov 2, 2015 at 10:42 PM, Tapas Chakrabarty <[email protected]>
>> wrote:
>>
>>> Ushadi,
>>> For molecular studies the herbarium specimens are not eligible as these
>>> are poisoned.  Sometimes back someone requested me to send fresh leaf
>>> samples  of Nicobariodendron sleumeri, tentatively placed under
>>> Celastraceae, for molecular studies to solve the problem whether it really
>>> belongs to the Celastraceae or a new family should be proposed.  Thus if
>>> Nicolas ji  can find out  some way of preserving his materials without
>>> poisoning, it is quite good but at the same time he should adhere  to the
>>> valuable  suggestions given by Pankaj ji.
>>> Regards,
>>> Tapas.
>>>
>>>
>>> On Mon, Nov 2, 2015 at 2:43 PM, Dr Pankaj Kumar <[email protected]>
>>> wrote:
>>>
>>>> Dear Sir
>>>> I am really fascinated to see these and other images on yoru website.
>>>> Ideally an image to be called a herbarium specimen, it needs to be in
>>>> the size of A3.
>>>> I noticed that none of your specimens have a number (voucher number).
>>>> Secondly, I would suggest you to try a black background. Just spread
>>>> your specimens on the scanner and scan removing the upper cover. The image
>>>> will have a black background but smaller flowers really look very nice. You
>>>> can still put the lid back and scan with white background.
>>>> Please also remember, if someday you need to describe a new species,
>>>> then you need a proper specimen and not the digital one. So also remember
>>>> to keep the sheet with dry plant always in your collection for reference.
>>>> Most of the real herbaria have old specimens that dates back to 300
>>>> years sometimes. They have their own importance and your work has its own,
>>>> so just keep going.
>>>> Best of luck.
>>>> If you need any help from me, please feel free to write to me here or
>>>> privately.
>>>> Thanks and regards
>>>> Pankaj
>>>>
>>>>
>>>>
>>>> On Thursday, 29 October 2015 18:05:01 UTC+8, Nicolas Lagarrigue wrote:
>>>>>
>>>>> Dear members of eflora of India group,
>>>>>
>>>>>
>>>>> We would like to share with you our new virtual herbarium, an online
>>>>> catalogue of plants from our region, including local, vulnerable and
>>>>> sometimes endangered species from the native evergreen forests of the
>>>>> Coromandel Coast of Tamil Nadu, as well as other widespread species of the
>>>>> Indian subcontinent.
>>>>>
>>>>>
>>>>> Unlike many other virtual herbaria, which use pictures of their
>>>>> existing collections, samples of which can be decades old, often
>>>>> discoloured and degraded, Pitchandikulam Forest Virtual Herbarium uses
>>>>> fresh specimens, briefly flattened and then scanned immediately in high
>>>>> definition, in order to conserve their natural colours and texture. This
>>>>> creates exquisite, vivid images which allow us to fully appreciate the
>>>>> intricate beauty of plants and to discover them more intimately.
>>>>>
>>>>>
>>>>> For each species, detailed botanical information is provided,
>>>>> including nomenclature, description, phenology, reproduction and 
>>>>> dispersal,
>>>>> as well details of plant ecology, distribution, conservation status and
>>>>> propagation and planting advice.
>>>>>
>>>>> In addition to the detailed scientific data, it offers additional
>>>>> ethnobotanical information (human uses of plants for food, crafts,
>>>>> medicines or rituals…).
>>>>>
>>>>>
>>>>> Pitchandikulam Forest Virtual Herbarium is a work in progress. So far
>>>>> 50 plant species are available to view and learn about, with new species
>>>>> added continuously.
>>>>>
>>>>> If you are interested, we encourage you to join our mailing list to
>>>>> get the latest updates.
>>>>>
>>>>>
>>>>> Pitchandikulam Forest Virtual Herbarium can be found here:
>>>>>
>>>>> www.pitchandikulam-herbarium.org
>>>>>
>>>>>
>>>>> Enjoy your visit and share it with all the plant lovers you know…
>>>>>
>>>>> Thank you.
>>>>>
>>>>> Irène and Nico
>>>>>
>>>>>
>>>>> <http://www.pitchandikulam-herbarium.org/img/mail_signature.jpg>
>>>>>
>>>>>
>>>>>
>>>>> <http://www.pitchandikulam-herbarium.org/img/pitchandikulam_herbarium.jpg>
>>>>>
>>>>>
>>>>> --
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>>>
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>>
>>
>>
>> --
>> Usha di
>> ===========
>>
>
>
>
> --
> ******************************************************
> ****************************************************************
> *Pankaj Kumar*, Ph.D.
> *IUCN-SSC Orchid Specialist Group Asia*
>
> *Office*:
>
> Conservation Officer
>
> Orchid Conservation Section
>
> Flora Conservation Department
>
> Kadoorie Farm and Botanic Garden (KFBG) Corporation
> Lam Kam Road, Tai Po, New Territories, Hong Kong.
>
> *Residence*:
> House no. 39, 2nd Floor, Shui Wo Tsuen
> Lam Tsuen, Tai Po, New Territories, Hong Kong.
> *email*: [email protected]; [email protected]
> *Phone*: +852 2483 7128 (office - 8:30am to 5:00pm); +852 9436 6251
> (mobile); *Fax*: +852 2483 7194
>
>


-- 
Usha di
===========

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