They say that microwave degerates the DNA and even protiens !! But havent seen any literature on it. Good thing to experiment...
On Wed, Nov 4, 2015 at 9:15 AM, Ushadi Micromini <[email protected]> wrote: > Thanks Pankaj > I had read it ( a few repeatedly) in some reviews or papers about the > molecular exams from old specimen > > I have also seen ( i was present when at ..... herbarium) some visiting > scientists were snipping small/minute leaf pieces from herbarium dried > leaf etc for exams ) > > could not have been for enzyme essays! > > usha di > > ps silica gel is how i dry my flowers for flower arranging for fall and > winter. > > would drying in microwave oven preserve the dna/r na structure?/ i have > not thought about it.. > is there any data out there? > > > On Wed, Nov 4, 2015 at 4:06 AM, Pankaj Kumar <[email protected]> > wrote: > >> Plant specimens were not taken for dna analysis earlier. But now a days >> they are. >> The main thing about DNA analysis is, it is best done from fresh samples >> or a sample which were dried without rotting (very fast drying). Hence we >> use silica gel for fast drying. As the samples on herbarium sheet or even >> in the silica gets older the chances of DNA extraction becomes less as the >> dna starts degenrating. >> Poison can be anything from mercury chloride or even alcohol. Infact at >> our organisation we dont use any chemicals to kill.but still we are not >> able to extract DNA !! >> >> On Tue, Nov 3, 2015 at 10:39 PM, Ushadi Micromini < >> [email protected]> wrote: >> >>> Tapasda >>> >>> poisoned by what ? >>> >>> mercury or something else? >>> >>> i thought some pieces of leaves are routinely taken for biochemical and >>> molecular analysis from herbarium specimen >>> >>> am i wrong? >>> >>> usha di >>> >>> >>> >>> >>> On Mon, Nov 2, 2015 at 10:42 PM, Tapas Chakrabarty <[email protected]> >>> wrote: >>> >>>> Ushadi, >>>> For molecular studies the herbarium specimens are not eligible as these >>>> are poisoned. Sometimes back someone requested me to send fresh leaf >>>> samples of Nicobariodendron sleumeri, tentatively placed under >>>> Celastraceae, for molecular studies to solve the problem whether it really >>>> belongs to the Celastraceae or a new family should be proposed. Thus if >>>> Nicolas ji can find out some way of preserving his materials without >>>> poisoning, it is quite good but at the same time he should adhere to the >>>> valuable suggestions given by Pankaj ji. >>>> Regards, >>>> Tapas. >>>> >>>> >>>> On Mon, Nov 2, 2015 at 2:43 PM, Dr Pankaj Kumar <[email protected] >>>> > wrote: >>>> >>>>> Dear Sir >>>>> I am really fascinated to see these and other images on yoru website. >>>>> Ideally an image to be called a herbarium specimen, it needs to be in >>>>> the size of A3. >>>>> I noticed that none of your specimens have a number (voucher number). >>>>> Secondly, I would suggest you to try a black background. Just spread >>>>> your specimens on the scanner and scan removing the upper cover. The image >>>>> will have a black background but smaller flowers really look very nice. >>>>> You >>>>> can still put the lid back and scan with white background. >>>>> Please also remember, if someday you need to describe a new species, >>>>> then you need a proper specimen and not the digital one. So also remember >>>>> to keep the sheet with dry plant always in your collection for reference. >>>>> Most of the real herbaria have old specimens that dates back to 300 >>>>> years sometimes. They have their own importance and your work has its own, >>>>> so just keep going. >>>>> Best of luck. >>>>> If you need any help from me, please feel free to write to me here or >>>>> privately. >>>>> Thanks and regards >>>>> Pankaj >>>>> >>>>> >>>>> >>>>> On Thursday, 29 October 2015 18:05:01 UTC+8, Nicolas Lagarrigue wrote: >>>>>> >>>>>> Dear members of eflora of India group, >>>>>> >>>>>> >>>>>> We would like to share with you our new virtual herbarium, an online >>>>>> catalogue of plants from our region, including local, vulnerable and >>>>>> sometimes endangered species from the native evergreen forests of the >>>>>> Coromandel Coast of Tamil Nadu, as well as other widespread species of >>>>>> the >>>>>> Indian subcontinent. >>>>>> >>>>>> >>>>>> Unlike many other virtual herbaria, which use pictures of their >>>>>> existing collections, samples of which can be decades old, often >>>>>> discoloured and degraded, Pitchandikulam Forest Virtual Herbarium uses >>>>>> fresh specimens, briefly flattened and then scanned immediately in high >>>>>> definition, in order to conserve their natural colours and texture. This >>>>>> creates exquisite, vivid images which allow us to fully appreciate the >>>>>> intricate beauty of plants and to discover them more intimately. >>>>>> >>>>>> >>>>>> For each species, detailed botanical information is provided, >>>>>> including nomenclature, description, phenology, reproduction and >>>>>> dispersal, >>>>>> as well details of plant ecology, distribution, conservation status and >>>>>> propagation and planting advice. >>>>>> >>>>>> In addition to the detailed scientific data, it offers additional >>>>>> ethnobotanical information (human uses of plants for food, crafts, >>>>>> medicines or rituals…). >>>>>> >>>>>> >>>>>> Pitchandikulam Forest Virtual Herbarium is a work in progress. So far >>>>>> 50 plant species are available to view and learn about, with new species >>>>>> added continuously. >>>>>> >>>>>> If you are interested, we encourage you to join our mailing list to >>>>>> get the latest updates. >>>>>> >>>>>> >>>>>> Pitchandikulam Forest Virtual Herbarium can be found here: >>>>>> >>>>>> www.pitchandikulam-herbarium.org >>>>>> >>>>>> >>>>>> Enjoy your visit and share it with all the plant lovers you know… >>>>>> >>>>>> Thank you. >>>>>> >>>>>> Irène and Nico >>>>>> >>>>>> >>>>>> <http://www.pitchandikulam-herbarium.org/img/mail_signature.jpg> >>>>>> >>>>>> >>>>>> >>>>>> <http://www.pitchandikulam-herbarium.org/img/pitchandikulam_herbarium.jpg> >>>>>> >>>>>> >>>>>> -- >>>>> You received this message because you are subscribed to the Google >>>>> Groups "efloraofindia" group. >>>>> To unsubscribe from this group and stop receiving emails from it, send >>>>> an email to [email protected]. >>>>> To post to this group, send email to [email protected]. >>>>> Visit this group at http://groups.google.com/group/indiantreepix. >>>>> For more options, visit https://groups.google.com/d/optout. >>>>> >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "efloraofindia" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to [email protected]. >>>> To post to this group, send email to [email protected]. >>>> Visit this group at http://groups.google.com/group/indiantreepix. >>>> For more options, visit https://groups.google.com/d/optout. >>>> >>> >>> >>> >>> -- >>> Usha di >>> =========== >>> >> >> >> >> -- >> ****************************************************** >> **************************************************************** >> *Pankaj Kumar*, Ph.D. >> *IUCN-SSC Orchid Specialist Group Asia* >> >> *Office*: >> >> Conservation Officer >> >> Orchid Conservation Section >> >> Flora Conservation Department >> >> Kadoorie Farm and Botanic Garden (KFBG) Corporation >> Lam Kam Road, Tai Po, New Territories, Hong Kong. >> >> *Residence*: >> House no. 39, 2nd Floor, Shui Wo Tsuen >> Lam Tsuen, Tai Po, New Territories, Hong Kong. >> *email*: [email protected]; [email protected] >> *Phone*: +852 2483 7128 (office - 8:30am to 5:00pm); +852 9436 6251 >> (mobile); *Fax*: +852 2483 7194 >> >> > > > -- > Usha di > =========== > -- ****************************************************** **************************************************************** *Pankaj Kumar*, Ph.D. *IUCN-SSC Orchid Specialist Group Asia* *Office*: Conservation Officer Orchid Conservation Section Flora Conservation Department Kadoorie Farm and Botanic Garden (KFBG) Corporation Lam Kam Road, Tai Po, New Territories, Hong Kong. *Residence*: House no. 39, 2nd Floor, Shui Wo Tsuen Lam Tsuen, Tai Po, New Territories, Hong Kong. *email*: [email protected]; [email protected] *Phone*: +852 2483 7128 (office - 8:30am to 5:00pm); +852 9436 6251 (mobile); *Fax*: +852 2483 7194 -- You received this message because you are subscribed to the Google Groups "efloraofindia" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send an email to [email protected]. Visit this group at http://groups.google.com/group/indiantreepix. For more options, visit https://groups.google.com/d/optout.
