Gurcharanji: you are right about the relative magnifications... you dont get to do million fold mag in SEm but thousands is possible... but can in TEM....
And I'll let in on a secret: if you can see 3D of the content ... shadows , fracture lines etc its SEM of a large specimen that had to be fixed or frozen etc and fractured/cut to show inside it... that's whats showing in fig 2 and 3 ... fig 1 is outright surface features that do not need cutting/fracturing... fig 4 is also SEM but not being up on botanical cellular structures at SEM level ...do not know what it is... Thanks Usha di ================= On Tue, Jan 10, 2012 at 3:44 PM, Gurcharan Singh <[email protected]> wrote: > Thanks Ushadi > May be I am wrong some where. I had read and taught my students that SEM > (which is generally of lower magnification than TEM) is meant for studying > of surface features such as epidermal cells, trichomes, whereas TEM are > details of cell contents such as chloroplast, nucleus, mitochondria, > protein bodies, crystals, etc. May be my knowledge is outdated. > > > -- > Dr. Gurcharan Singh > Retired Associate Professor > SGTB Khalsa College, University of Delhi, Delhi-110007 > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. > Phone: 011-25518297 Mob: 9810359089 > http://people.du.ac.in/~singhg45/ > > > > On Tue, Jan 10, 2012 at 3:08 PM, Ushadi micromini < > [email protected]> wrote: > >> Gurcharanji: >> I dont mean to correct you... >> but all are SEM..... >> >> not a single one of these is a TEM.... >> >> Usha di >> ========== >> >> >> >> >> >> On Jan 10, 1:52 pm, Gurcharan Singh <[email protected]> wrote: >> > Thanks Pankaj ji and Ushadi >> > I read second mail of Vijay ji and somehow missed looking at >> attachments. >> > Thanks for bringing it to my notice. >> > >> > Should we say >> > First picture is SEM of leaf showing trichomes >> > Second and third picture are TEM of cells showing crystals >> > 4th again seems to be SEM >> > >> > -- >> > Dr. Gurcharan Singh >> > Retired Associate Professor >> > SGTB Khalsa College, University of Delhi, Delhi-110007 >> > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >> > Phone: 011-25518297 Mob: 9810359089http://people.du.ac.in/~singhg45/ >> > >> > On Tue, Jan 10, 2012 at 2:11 PM, Ushadi micromini < >> [email protected] >> >> > >> > >> > >> > >> > >> > >> > >> > > wrote: >> > >> > > Gurcharanji: >> > > if you go to abridged summary that came this morning and go to the >> > > first email... its from Raman... and its title is :" My Flora >> > > Picture of the Year 2011: Vijayasankar Raman " >> > >> > > there you scroll down ... and just above my first comment ... are the >> > > SEM pics ... i was asking about... >> > >> > > Is n't it wonderful.... >> > > ??? >> > >> > > love it >> > > Usha di >> > > ============ >> > >> > > On Jan 10, 1:37 pm, ushadi Micromini <[email protected]> >> > > wrote: >> > > > Thanks Pankaj... >> > > > crystals I could tell... >> > >> > > > My q to Vijaya was / is : is it cell wall... that's so rigid and >> forming >> > > > the rhomboid spongy looking mesh... >> > >> > > > usha di >> > > > ======== >> > >> > > > On Tue, Jan 10, 2012 at 1:55 PM, Pankaj Kumar < >> [email protected] >> > > >wrote: >> > >> > > > > Mam, 2nd and third pics show crystals (raphides) inside the cell. >> > > > > These crystals as well as their structure sometimes form a good >> > > > > taxonomic character of groups. >> > > > > Pankaj >> > >> > > > > On Tue, Jan 10, 2012 at 4:19 PM, Ushadi micromini >> > > > > <[email protected]> wrote: >> > > > > > Dear Vijayashankar: >> > >> > > > > > A I am so excited ... >> > >> > > > > > 1: first for you, this will add some new or deeper insights >> to >> > > > > > your ongoing studies.... but of course you knew that...! >> > > > > > 2: I am excited for myself and the whole of eflora >> family... >> > > > > > we will get to see the botanical specimen in further details... >> as >> > > and >> > > > > > when you share your finds... >> > >> > > > > > ================== >> > >> > > > > > B: >> > >> > > > > > Now, you will be Vijyashankar micromini .... >> > > > > > instead of / in addition to me... >> > >> > > > > > the micromini epithet was attached to my name by a few friends >> and >> > > > > > colleagues a couple or three decades ago because I was fond of >> doing >> > > > > > TEM and SEM of tumors etc....and ribosome...and mitochondria... >> > >> > > > > > ======== >> > >> > > > > > C: and this the question section :-) >> > >> > > > > > the first pic is surface of leaf..... i imagine.... >> > >> > > > > > but what are 2 and 3rd pictures of ... a fracture of what? >> > >> > > > > > is it of a leaf and the rhoboid? structures cell walls... with >> > > > > > crystals etc inside? >> > >> > > > > > ============== >> > >> > > > > > just cant stop smiling.... what a great new dimension to our >> > > > > > interaction will this make.... >> > > > > > wonderful for all of us... >> > >> > > > > > and congratulations for learning the techniques... >> > > > > > ushadi >> > >> > > > > > ========== >> > > > > > ps does this grow in mississipi??? >> > >> > > > > > ================= >> > >> > > > > > On Jan 10, 12:44 pm, "J.M. Garg" <[email protected]> wrote: >> > > > > >> Thanks, Vijayasankar ji, >> > > > > >> Wonderly different idea & picture. >> > >> > > > > >> For others: >> > > > > >> Scanning Electron Microscopy (SEM): >> > > > > >> http://serc.carleton.edu/research_education/geochemsheets/techniques/. >> > > .. >> > >> > > > > >> A link for Scanning electron microscope: >> > > > >http://en.wikipedia.org/wiki/Scanning_electron_microscope >> > >> > > > > >> On 10 January 2012 05:15, Vijayasankar < >> [email protected]> >> > > > > wrote: >> > >> > > > > >> > Dear all >> > >> > > > > >> > Here is yet another b&w picture as 'my flora picture of the >> year >> > > > > 2011'. >> > > > > >> > I am not sure if this is suitable for this title, though. >> > >> > > > > >> > But this is my first ever SEM picture :) It was my >> > > dream....finally >> > > > > came >> > > > > >> > true! It was a great experience to learn the techniques >> without a >> > > > > tutor >> > > > > >> > (but now I am helping many...). >> > > > > >> > The picture shows glandular and non-glandular trichomes from >> leaf >> > > of >> > > > > >> > Salvia divinorum, a psychoactive plant endemic to Mexico. >> > > > > >> > I recorded 5 types and 33 sub-types of trichomes (some of >> them >> > > are new >> > > > > >> > reports), from a single leaf, thanks to SEM... >> > >> > > > > >> > Regards >> > >> > > > > >> > Vijayasankar Raman >> > > > > >> > National Center for Natural Products Research >> > > > > >> > University of Mississippi >> > >> > > > > >> -- >> > > > > >> With regards, >> > > > > >> J.M.Garg ([email protected]) >> > >http://en.wikipedia.org/wiki/User:Jmgarg1 >> > > > > >> 'Creating awareness of Indian Flora & Fauna' >> > > > > >> The whole world uses my Image Resource of more than a *thousand >> > > > > species* & >> > > > > >> eight thousand images of Birds, Butterflies, Plants etc. >> (arranged >> > > > > >> alphabetically & place-wise): >> > > > >http://commons.wikimedia.org/wiki/Category:J.M.Garg. You can also >> use >> > > them >> > > > > >> for free as per Creative Commons license attached with each >> image. >> > > > > >> For identification, learning, discussion & documentation of >> Indian >> > > > > Flora, >> > > > > >> please visit/ join our Efloraofindia Google e-group: >> > > > >http://groups.google.co.in/group/indiantreepix(morethan1760members & >> >> > > > > >> 1,00,000 messages on 21/12/11) or Efloraofindia website: >> > > > >https://sites.google.com/site/efloraofindia/(withaspecies database >> >> > > > > >> of more than 6000 species). >> > > > > >> Also author of 'A Photoguide to the Birds of Kolkata & Common >> Birds >> > > of >> > > > > >> India'. >> > >> > > > > -- >> > > > > >> ********************************************************************** >> > > > > "Taxonomists getting Extinct and Species Data Deficient !!" >> > >> > > > > Pankaj Kumar Ph.D. (Orchidaceae) >> > > > > Conservation Officer >> > >> > > > > Office: >> > > > > Orchid Conservation Section >> > > > > Flora Conservation Department >> > > > > Kadoorie Farm and Botanic Garden (KFBG) Corporation >> > > > > Lam Kam Road, Tai Po, New Territories, Hong Kong. >> > >> > > > > Residence: >> > > > > 36c, Ng Tung Chai, Lam Tseun >> > > > > Tai Po, New Territories, Hong Kong. >> > >> > > > > email: [email protected] >> > > > > [email protected] >> > > > > [email protected] >> > > > > Phone: +852 2483 7128 (office - 8:30am to 5:30pm) >> > > > > +852 9436 6251 (mobile) >> > >> > > > -- >> > > > Usha di >> > > > =========== >> > > > > -- Usha di ===========
