I feel this is my 'eflora thread of the year 2011' ! I am delighted to see the overwhelming responses from you all. Thanks to one and all for your kind words and appreciations.
Dear Gurcharan ji, all the four pictures are by SEM only. Even I was thinking that SEM was used only to study the surface features until recently when I read an article in which anatomy of a fern using SEM was published. So I decided to try it out and I got very encouraging results (of course after several trial and errors :). I hope to share the paper soon with you all. Dear Ushadi, nice to know the meaning of your 'epithet' name. And I am happy in joining your 'family'. Thanks for all your nice words. The first picture is the under (abaxial) surface of leaf. 2nd and 3rd are of T.S. of petiole with crystals in the parenchyma / idioblasts cells. The plant has different types of crystals incl. prisms and raphides. The 4th picture is of a peltate glandular trichome that contains the psychoactive alkaloid Salvinorin-A. Our SEM is capable of magnifying up to 300,000 x Regards Vijayasankar Raman National Center for Natural Products Research University of Mississippi On Tue, Jan 10, 2012 at 4:18 AM, Prashant Awale <[email protected]> wrote: > Dear Vijayasankar ji, > > I really liked this post. Its really a "divine feeling." Thanks for this > unique post. > Regards > Prashant > On Tue, Jan 10, 2012 at 3:08 PM, Ushadi micromini < > [email protected]> wrote: > >> Gurcharanji: >> I dont mean to correct you... >> but all are SEM..... >> >> not a single one of these is a TEM.... >> >> Usha di >> ========== >> >> >> >> >> On Jan 10, 1:52 pm, Gurcharan Singh <[email protected]> wrote: >> > Thanks Pankaj ji and Ushadi >> > I read second mail of Vijay ji and somehow missed looking at >> attachments. >> > Thanks for bringing it to my notice. >> > >> > Should we say >> > First picture is SEM of leaf showing trichomes >> > Second and third picture are TEM of cells showing crystals >> > 4th again seems to be SEM >> > >> > -- >> > Dr. Gurcharan Singh >> > Retired Associate Professor >> > SGTB Khalsa College, University of Delhi, Delhi-110007 >> > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >> > Phone: 011-25518297 Mob: 9810359089http://people.du.ac.in/~singhg45/ >> > >> > On Tue, Jan 10, 2012 at 2:11 PM, Ushadi micromini < >> [email protected] >> > >> > >> > >> > >> > >> > >> > >> > > wrote: >> > >> > > Gurcharanji: >> > > if you go to abridged summary that came this morning and go to the >> > > first email... its from Raman... and its title is :" My Flora >> > > Picture of the Year 2011: Vijayasankar Raman " >> > >> > > there you scroll down ... and just above my first comment ... are the >> > > SEM pics ... i was asking about... >> > >> > > Is n't it wonderful.... >> > > ??? >> > >> > > love it >> > > Usha di >> > > ============ >> > >> > > On Jan 10, 1:37 pm, ushadi Micromini <[email protected]> >> > > wrote: >> > > > Thanks Pankaj... >> > > > crystals I could tell... >> > >> > > > My q to Vijaya was / is : is it cell wall... that's so rigid and >> forming >> > > > the rhomboid spongy looking mesh... >> > >> > > > usha di >> > > > ======== >> > >> > > > On Tue, Jan 10, 2012 at 1:55 PM, Pankaj Kumar < >> [email protected] >> > > >wrote: >> > >> > > > > Mam, 2nd and third pics show crystals (raphides) inside the cell. >> > > > > These crystals as well as their structure sometimes form a good >> > > > > taxonomic character of groups. >> > > > > Pankaj >> > >> > > > > On Tue, Jan 10, 2012 at 4:19 PM, Ushadi micromini >> > > > > <[email protected]> wrote: >> > > > > > Dear Vijayashankar: >> > >> > > > > > A I am so excited ... >> > >> > > > > > 1: first for you, this will add some new or deeper insights >> to >> > > > > > your ongoing studies.... but of course you knew that...! >> > > > > > 2: I am excited for myself and the whole of eflora >> family... >> > > > > > we will get to see the botanical specimen in further details... >> as >> > > and >> > > > > > when you share your finds... >> > >> > > > > > ================== >> > >> > > > > > B: >> > >> > > > > > Now, you will be Vijyashankar micromini .... >> > > > > > instead of / in addition to me... >> > >> > > > > > the micromini epithet was attached to my name by a few friends >> and >> > > > > > colleagues a couple or three decades ago because I was fond of >> doing >> > > > > > TEM and SEM of tumors etc....and ribosome...and mitochondria... >> > >> > > > > > ======== >> > >> > > > > > C: and this the question section :-) >> > >> > > > > > the first pic is surface of leaf..... i imagine.... >> > >> > > > > > but what are 2 and 3rd pictures of ... a fracture of what? >> > >> > > > > > is it of a leaf and the rhoboid? structures cell walls... with >> > > > > > crystals etc inside? >> > >> > > > > > ============== >> > >> > > > > > just cant stop smiling.... what a great new dimension to our >> > > > > > interaction will this make.... >> > > > > > wonderful for all of us... >> > >> > > > > > and congratulations for learning the techniques... >> > > > > > ushadi >> > >> > > > > > ========== >> > > > > > ps does this grow in mississipi??? >> > >> > > > > > ================= >> > >> > > > > > On Jan 10, 12:44 pm, "J.M. Garg" <[email protected]> wrote: >> > > > > >> Thanks, Vijayasankar ji, >> > > > > >> Wonderly different idea & picture. >> > >> > > > > >> For others: >> > > > > >> Scanning Electron Microscopy (SEM): >> > > > > >> http://serc.carleton.edu/research_education/geochemsheets/techniques/. >> > > .. >> > >> > > > > >> A link for Scanning electron microscope: >> > > > >http://en.wikipedia.org/wiki/Scanning_electron_microscope >> > >> > > > > >> On 10 January 2012 05:15, Vijayasankar < >> [email protected]> >> > > > > wrote: >> > >> > > > > >> > Dear all >> > >> > > > > >> > Here is yet another b&w picture as 'my flora picture of the >> year >> > > > > 2011'. >> > > > > >> > I am not sure if this is suitable for this title, though. >> > >> > > > > >> > But this is my first ever SEM picture :) It was my >> > > dream....finally >> > > > > came >> > > > > >> > true! It was a great experience to learn the techniques >> without a >> > > > > tutor >> > > > > >> > (but now I am helping many...). >> > > > > >> > The picture shows glandular and non-glandular trichomes from >> leaf >> > > of >> > > > > >> > Salvia divinorum, a psychoactive plant endemic to Mexico. >> > > > > >> > I recorded 5 types and 33 sub-types of trichomes (some of >> them >> > > are new >> > > > > >> > reports), from a single leaf, thanks to SEM... >> > >> > > > > >> > Regards >> > >> > > > > >> > Vijayasankar Raman >> > > > > >> > National Center for Natural Products Research >> > > > > >> > University of Mississippi >> > >> > > > > >> -- >> > > > > >> With regards, >> > > > > >> J.M.Garg ([email protected]) >> > >http://en.wikipedia.org/wiki/User:Jmgarg1 >> > > > > >> 'Creating awareness of Indian Flora & Fauna' >> > > > > >> The whole world uses my Image Resource of more than a *thousand >> > > > > species* & >> > > > > >> eight thousand images of Birds, Butterflies, Plants etc. >> (arranged >> > > > > >> alphabetically & place-wise): >> > > > >http://commons.wikimedia.org/wiki/Category:J.M.Garg. You can also >> use >> > > them >> > > > > >> for free as per Creative Commons license attached with each >> image. >> > > > > >> For identification, learning, discussion & documentation of >> Indian >> > > > > Flora, >> > > > > >> please visit/ join our Efloraofindia Google e-group: >> > > > >http://groups.google.co.in/group/indiantreepix(morethan1760members & >> > > > > >> 1,00,000 messages on 21/12/11) or Efloraofindia website: >> > > > >https://sites.google.com/site/efloraofindia/(withaspecies database >> > > > > >> of more than 6000 species). >> > > > > >> Also author of 'A Photoguide to the Birds of Kolkata & Common >> Birds >> > > of >> > > > > >> India'. >> > >> > > > > -- >> > > > > >> ********************************************************************** >> > > > > "Taxonomists getting Extinct and Species Data Deficient !!" >> > >> > > > > Pankaj Kumar Ph.D. (Orchidaceae) >> > > > > Conservation Officer >> > >> > > > > Office: >> > > > > Orchid Conservation Section >> > > > > Flora Conservation Department >> > > > > Kadoorie Farm and Botanic Garden (KFBG) Corporation >> > > > > Lam Kam Road, Tai Po, New Territories, Hong Kong. >> > >> > > > > Residence: >> > > > > 36c, Ng Tung Chai, Lam Tseun >> > > > > Tai Po, New Territories, Hong Kong. >> > >> > > > > email: [email protected] >> > > > > [email protected] >> > > > > [email protected] >> > > > > Phone: +852 2483 7128 (office - 8:30am to 5:30pm) >> > > > > +852 9436 6251 (mobile) >> > >> > > > -- >> > > > Usha di >> > > > =========== >> > >
