Dear Chitra, Do you want to analyse the sugar concentration in nectar? If so, you may use any handheld refractometer (Model-ABT-32, VEE GEE Scientific, Kirkland, WA, USA, is an example), that you can carry to the field and determine the sugar level of nectar in flowers. You can collect nectar from flowers of different sizes by using capillaries of different sizes starting from 0.5µl to 100µl. You can select capillaries of different size according to the size (diameter) of the corolla tube (not the diameter of the flower).
Regards, Giby On 2 July 2012 11:49, Chitra Shanker <[email protected]> wrote: > I have a query to all professional botanists on this forum. Which > technique is best used for determination of sucrose, glucose and fructose > content in flowers especially really small ones > > -- > Dr. Chitra Shanker > Sr. Scientist (Entomology) > Directorate of Rice Research, > Rajendranagar, Hyderabad -500030 > > > -- GIBY KURIAKOSE PhD Ashoka Trust for Research in Ecology and the Environment (ATREE), Royal Enclave, Jakkur Post, Srirampura Bangalore- 560064 India Phone - +91 9448714856 +919947109987 (Mobile) visit my pictures @ http://www.flickr.com/photos/giby
