Forwarding again for any assistance in the matter please.
---------- Forwarded message ---------- From: Chitra Shanker <[email protected]> Date: 2 July 2012 13:23 Subject: Re: [efloraofindia:120770] A query To: Giby Kuriakose <[email protected]> Cc: indiantreepix <[email protected]> Thank you Giby for th reply.I do have the procedure for nectar collection. But I neither hav capillaries nor the refractometer. Neither can I do the HPLC which is quoted in many recent references. But I have reference for rinsing flowers and the van hendel method (anthrone) for sucrose. I would like to know is this this procedure used often. Chitra On Mon, Jul 2, 2012 at 1:15 PM, Giby Kuriakose <[email protected]>wrote: > Dear Chitra, > > Do you want to analyse the sugar concentration in nectar? > If so, you may use any handheld refractometer (Model-ABT-32, VEE GEE > Scientific, Kirkland, WA, USA, is an example), that you can carry to the > field and determine the sugar level of nectar in flowers. You can collect > nectar from flowers of different sizes by using capillaries of different > sizes starting from 0.5µl to 100µl. You can select capillaries of > different size according to the size (diameter) of the corolla tube (not > the diameter of the flower). > > > Regards, > Giby > > > > > > On 2 July 2012 11:49, Chitra Shanker <[email protected]> wrote: > >> I have a query to all professional botanists on this forum. Which >> technique is best used for determination of sucrose, glucose and fructose >> content in flowers especially really small ones >> >> -- >> Dr. Chitra Shanker >> Sr. Scientist (Entomology) >> Directorate of Rice Research, >> Rajendranagar, Hyderabad -500030 >> >> >> > > > -- > GIBY KURIAKOSE PhD > Ashoka Trust for Research in Ecology and the Environment (ATREE), > Royal Enclave, > Jakkur Post, Srirampura > Bangalore- 560064 > India > Phone - +91 9448714856 +919947109987 (Mobile) > visit my pictures @ http://www.flickr.com/photos/giby > -- Dr. Chitra Shanker Sr. Scientist (Entomology) Directorate of Rice Research, Rajendranagar, Hyderabad -500030 -- With regards, J.M.Garg http://en.wikipedia.org/wiki/User:Jmgarg1 'Creating awareness of Indian Flora & Fauna' The whole world uses my Image Resource of more than a *thousand species* & eight thousand images of Birds, Butterflies, Plants etc. (arranged alphabetically & place-wise): http://commons.wikimedia.org/wiki/Category:J.M.Garg. You can also use them for free as per Creative Commons license attached with each image. For identification, learning, discussion & documentation of Indian Flora, please visit/ join our Efloraofindia Google e-group: http://groups.google.co.in/group/indiantreepix (more than 1900 members & 1,20,700 messages on 30/6/12) or Efloraofindia website: https://sites.google.com/site/efloraofindia/ (with a species database of more than 7000 species). Also author of 'A Photoguide to the Birds of Kolkata & Common Birds of India'.
