Thank you Giby for th reply.I do have the procedure for nectar collection. But I neither hav capillaries nor the refractometer. Neither can I do the HPLC which is quoted in many recent references. But I have reference for rinsing flowers and the van hendel method (anthrone) for sucrose. I would like to know is this this procedure used often. Chitra
On Mon, Jul 2, 2012 at 1:15 PM, Giby Kuriakose <[email protected]>wrote: > Dear Chitra, > > Do you want to analyse the sugar concentration in nectar? > If so, you may use any handheld refractometer (Model-ABT-32, VEE GEE > Scientific, Kirkland, WA, USA, is an example), that you can carry to the > field and determine the sugar level of nectar in flowers. You can collect > nectar from flowers of different sizes by using capillaries of different > sizes starting from 0.5µl to 100µl. You can select capillaries of > different size according to the size (diameter) of the corolla tube (not > the diameter of the flower). > > > Regards, > Giby > > > > > > On 2 July 2012 11:49, Chitra Shanker <[email protected]> wrote: > >> I have a query to all professional botanists on this forum. Which >> technique is best used for determination of sucrose, glucose and fructose >> content in flowers especially really small ones >> >> -- >> Dr. Chitra Shanker >> Sr. Scientist (Entomology) >> Directorate of Rice Research, >> Rajendranagar, Hyderabad -500030 >> >> >> > > > -- > GIBY KURIAKOSE PhD > Ashoka Trust for Research in Ecology and the Environment (ATREE), > Royal Enclave, > Jakkur Post, Srirampura > Bangalore- 560064 > India > Phone - +91 9448714856 +919947109987 (Mobile) > visit my pictures @ http://www.flickr.com/photos/giby > -- Dr. Chitra Shanker Sr. Scientist (Entomology) Directorate of Rice Research, Rajendranagar, Hyderabad -500030
