Hi Cristina, glad to hear that v2.4.0 enabled you to get past the OS X security nanny issues.
As for the type of alignments you are trying to construct, I am afraid that it's likely to to be very resource intensive with the progressiveMauve algorithm, especially for RAM and CPU time. It is certainly possible to align 35 bacterial genomes with progressiveMauve and others have aligned many more, but I would take a rough guess that you will need a machine with 32 or 64GB of RAM. If you've got draft genomes, reordering the contigs relative to a single reference will help reduce memory demands for the subsequent multiple alignment. As for the problem on windows, would you be able to send a bit more of the text preceding the exit error message? It would help to know what the aligner was doing right before it crashed. Best to send the whole log if possible, although if it's big then maybe better to email it to me off-list. Cheers, -Aaron On Thu, 2015-01-22 at 16:33 +1100, cav00004 wrote: > Dear Aaron, > > Thank you for your quick reply. > I simply downloaded the new version v2.4.0 and it seems to work properly. I > lunched yesterday an alignment of 16 bacterial g > enomes, about 3Gb each, and is still working... so is great. I think now the > only limitation is the RAM memory as I use my p > ersonal computer and is not very performant: > > - MacBook Pro (13 inch, late 2011) > - Processor 2,4 GHz Inter Core i5 > - Memory 4 Go 1333 MHz DDR3 > - Graphics Inter HD Graphics 3000 384 Mo > > I will need to add another 19 sequences to the alignment, so a total of 35 > bacterial genomes. Do you think I will have any t > rouble? Will Mauve have difficulties to align such a big number of sequences? > > In the other hand, I was intended to work with the PC at the laboratory, a > common PC, and I had no problem recently, but sud > denly it appeared an error message after just lunching the alignment. I > downloaded the new version as well for Windows, but > the problem was not solved. The error message is the following: > > Error: mauve Aligner exited with and error code. Check the log window for > details. > > When checking the log window (Mauve Console?), the last line says: > "This application has requested the Runtime to terminate it in an unusual > way. > Please contact the application's support team for more information. > Exited with error code: 3" > > Please, can you help me to solve the problem with the Windows version? > > Thank you very much in advance > > Cristina > > > ------------------------ > Cristina Andres-Barrao, PhD. > cav00...@nodai.ac.jp > Laboratory of Fermentation Biochemistry > Department of Fermentation Sciences > Faculty of Applied Biosciences > Tokyo University of Agriculture > 1-1-1 Sakuragaoka, Setagaya-ku > Tokyo 156-8502, Japan > > ------------------------------------------------------------------------------ > New Year. New Location. New Benefits. New Data Center in Ashburn, VA. > GigeNET is offering a free month of service with a new server in Ashburn. > Choose from 2 high performing configs, both with 100TB of bandwidth. > Higher redundancy.Lower latency.Increased capacity.Completely compliant. > http://p.sf.net/sfu/gigenet > _______________________________________________ > Mauve-users mailing list > Mauve-users@lists.sourceforge.net > https://lists.sourceforge.net/lists/listinfo/mauve-users -- Aaron E. Darling, Ph.D. Associate Professor, ithree institute University of Technology Sydney Australia http://darlinglab.org twitter: @koadman UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. ------------------------------------------------------------------------------ New Year. New Location. New Benefits. New Data Center in Ashburn, VA. GigeNET is offering a free month of service with a new server in Ashburn. Choose from 2 high performing configs, both with 100TB of bandwidth. Higher redundancy.Lower latency.Increased capacity.Completely compliant. http://p.sf.net/sfu/gigenet _______________________________________________ Mauve-users mailing list Mauve-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/mauve-users
