Dear Aaron,

Thank you for your quick reply. 
I simply downloaded the new version v2.4.0 and it seems to work properly. I 
lunched yesterday an alignment of 16 bacterial g
enomes, about 3Gb each, and is still working... so is great. I think now the 
only limitation is the RAM memory as I use my p
ersonal computer and is not very performant:

   - MacBook Pro (13 inch, late 2011)
   - Processor 2,4 GHz Inter Core i5
   - Memory 4 Go 1333 MHz DDR3 
   - Graphics Inter HD Graphics 3000 384 Mo

I will need to add another 19 sequences to the alignment, so a total of 35 
bacterial genomes. Do you think I will have any t
rouble? Will Mauve have difficulties to align such a big number of sequences?

In the other hand, I was intended to work with the PC at the laboratory, a 
common PC, and I had no problem recently, but sud
denly it appeared an error message after just lunching the alignment. I 
downloaded the new version as well for Windows, but 
the problem was not solved. The error message is the following:

   Error: mauve Aligner exited with and error code. Check the log window for 
details.

When checking the log window (Mauve Console?), the last line says:
   "This application has requested the Runtime to terminate it in an unusual 
way.
   Please contact the application's support team for more information.
   Exited with error code: 3"

Please, can you help me to solve the problem with the Windows version?

Thank you very much in advance

Cristina


------------------------
Cristina Andres-Barrao, PhD.
cav00...@nodai.ac.jp
Laboratory of Fermentation Biochemistry
Department of Fermentation Sciences
Faculty of Applied Biosciences
Tokyo University of Agriculture
1-1-1 Sakuragaoka, Setagaya-ku
Tokyo 156-8502, Japan

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