Hi, I’ve some commentaries: > > you will need a machine with 32 or 64GB of RAM. If you've got draft
I aligned successfully 42 bacterial genomes (S. aureus) from the same genotype, if I use different genotypes, I can align about 15 genomes with a MacPro with 32 GB of RAM > genomes, reordering the contigs relative to a single reference will help > reduce memory demands for the subsequent multiple alignment. for me it’s not the case: progmauve works better with unordered genomes for me! (What it is really not logic for me, Aaron, I think that I have send you my genomes) All the best, Tom > > As for the problem on windows, would you be able to send a bit more of > the text preceding the exit error message? It would help to know what > the aligner was doing right before it crashed. Best to send the whole > log if possible, although if it's big then maybe better to email it to > me off-list. > > Cheers, > -Aaron > > > On Thu, 2015-01-22 at 16:33 +1100, cav00004 wrote: >> Dear Aaron, >> >> Thank you for your quick reply. >> I simply downloaded the new version v2.4.0 and it seems to work properly. I >> lunched yesterday an alignment of 16 bacterial g >> enomes, about 3Gb each, and is still working... so is great. I think now the >> only limitation is the RAM memory as I use my p >> ersonal computer and is not very performant: >> >> - MacBook Pro (13 inch, late 2011) >> - Processor 2,4 GHz Inter Core i5 >> - Memory 4 Go 1333 MHz DDR3 >> - Graphics Inter HD Graphics 3000 384 Mo >> >> I will need to add another 19 sequences to the alignment, so a total of 35 >> bacterial genomes. Do you think I will have any t >> rouble? Will Mauve have difficulties to align such a big number of sequences? >> >> In the other hand, I was intended to work with the PC at the laboratory, a >> common PC, and I had no problem recently, but sud >> denly it appeared an error message after just lunching the alignment. I >> downloaded the new version as well for Windows, but >> the problem was not solved. The error message is the following: >> >> Error: mauve Aligner exited with and error code. Check the log window for >> details. >> >> When checking the log window (Mauve Console?), the last line says: >> "This application has requested the Runtime to terminate it in an unusual >> way. >> Please contact the application's support team for more information. >> Exited with error code: 3" >> >> Please, can you help me to solve the problem with the Windows version? >> >> Thank you very much in advance >> >> Cristina >> >> >> ------------------------ >> Cristina Andres-Barrao, PhD. >> cav00...@nodai.ac.jp >> Laboratory of Fermentation Biochemistry >> Department of Fermentation Sciences >> Faculty of Applied Biosciences >> Tokyo University of Agriculture >> 1-1-1 Sakuragaoka, Setagaya-ku >> Tokyo 156-8502, Japan >> >> ------------------------------------------------------------------------------ >> New Year. New Location. New Benefits. New Data Center in Ashburn, VA. >> GigeNET is offering a free month of service with a new server in Ashburn. >> Choose from 2 high performing configs, both with 100TB of bandwidth. >> Higher redundancy.Lower latency.Increased capacity.Completely compliant. >> http://p.sf.net/sfu/gigenet >> _______________________________________________ >> Mauve-users mailing list >> Mauve-users@lists.sourceforge.net >> https://lists.sourceforge.net/lists/listinfo/mauve-users > > -- > Aaron E. Darling, Ph.D. > Associate Professor, ithree institute > University of Technology Sydney > Australia > > http://darlinglab.org > twitter: @koadman > > > > > > UTS CRICOS Provider Code: 00099F > DISCLAIMER: This email message and any accompanying attachments may contain > confidential information. > If you are not the intended recipient, do not read, use, disseminate, > distribute or copy this message or > attachments. If you have received this message in error, please notify the > sender immediately and delete > this message. Any views expressed in this message are those of the individual > sender, except where the > sender expressly, and with authority, states them to be the views of the > University of Technology Sydney. > Before opening any attachments, please check them for viruses and defects. > > Think. Green. Do. > > Please consider the environment before printing this email. > ------------------------------------------------------------------------------ > New Year. New Location. New Benefits. New Data Center in Ashburn, VA. > GigeNET is offering a free month of service with a new server in Ashburn. > Choose from 2 high performing configs, both with 100TB of bandwidth. > Higher redundancy.Lower latency.Increased capacity.Completely compliant. > http://p.sf.net/sfu/gigenet > _______________________________________________ > Mauve-users mailing list > Mauve-users@lists.sourceforge.net > https://lists.sourceforge.net/lists/listinfo/mauve-users ------------------------------------------------------------------------------ New Year. New Location. New Benefits. New Data Center in Ashburn, VA. GigeNET is offering a free month of service with a new server in Ashburn. Choose from 2 high performing configs, both with 100TB of bandwidth. Higher redundancy.Lower latency.Increased capacity.Completely compliant. http://p.sf.net/sfu/gigenet _______________________________________________ Mauve-users mailing list Mauve-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/mauve-users
