Thanks for the reply Aaron...I actually have admin privileges and checked
my permissions and have read/write....nonetheless I tried an alignment and
pointed the saved output to the desktop (test).....it looked like it went
farther but only an empty 'test' file ended up on the desktop:
Running alignment.
Executing
win64\progressiveMauve
--output=C:\Users\dboyd\Desktop\test
--output-guide-tree=C:\Users\dboyd\Desktop\test.guide_tree
--backbone-output=C:\Users\dboyd\Desktop\test.backbone
L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAM04528.gbk
L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAR17.gbk
Storing raw sequence at C:\Users\dboyd\AppData\Local\Temp\rawEAEC.tmp
Sequence loaded successfully.
L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAM04528.gbk 158213 base
pairs.
Storing raw sequence at C:\Users\dboyd\AppData\Local\Temp\rawEB3B.tmp
Sequence loaded successfully.
L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAR17.gbk 143963 base
pairs.
Using weight 13 mers for initial seeds
Creating sorted mer list
Create time was: 0 seconds.
Creating sorted mer list
Create time was: 1 seconds.
3%..6%..9%..13%..
16%..19%..23%..
26%..29%..33%..
36%..39%..43%..
46%..49%..52%..
56%..59%..62%..
66%..69%..72%..
76%..79%..82%..
86%..89%..92%..
95%..97%..99%..done.
using default bp penalty: 120435
using default bp estimate min score: 361306
Starting with 1332 multi-matches
Computing genome content distance matrix...
Genome conservation distance matrix:
0 0.394941
0.394941 0
Writing guide tree to C:\Users\dboyd\Desktop\test.guide_tree
Exited with error code: -1073741819
Dave
*******************************************************************************************************************
Hi David, intermingled with the normal output are some error messages
indicating Mauve isn't able to write to the output location you've
specified:
"Unable to open output file "test6" for writing."
I would suggest that you try the same alignment again, but take care to
use the output file chooser (the button with ... in the GUI) to select a
location to which you have write access. The desktop or your documents
folder are some candidates.
Best,
-Aaron
On Fri, 2015-02-13 at 08:54 +1100, David Boyd wrote:
> I first downloaded Mauve 2.4 in mid-Dec. and it worked fine (Windows 7
> Pro, 64 bit). Didn't try to use it again until a few weeks ago and
> just will not work. Tried some a couple of gbk and a couple of fas
> files. I can look at old alignments on 2.4.0 that were done in 2.3.1.
>
> Running alignment.
> Executing
> win64\progressiveMauve
> --output=test6
> --output-guide-tree=test6.guide_tree
> --backbone-output=test6.backbone
> L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAM04528.gbk
> L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAR17.gbk
> Storing raw sequence at C:\Users\dboyd\AppData\Local\Temp\rawD672.tmp
> Sequence loaded successfully.
> L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAM04528.gbk 158213
> base pairs.
> Storing raw sequence at C:\Users\dboyd\AppData\Local\Temp\rawD683.tmp
> Sequence loaded successfully.Unable to open output file "test6" for
> writing.
>
> L:\Nosocomial\DAB\PlasmidSequencing\IncACplasmids\pAR17.gbk 143963
> base pairs.Check that you have permission to write files in this
> location and that the disk has free space.
>
> Using weight 13 mers for initial seeds
>
> This application has requested the Runtime to terminate it in an
> unusual way.Creating sorted mer list
>
> Please contact the application's support team for more
> information.Create time was: 0 seconds.
>
> Creating sorted mer list
> Create time was: 0 seconds.
> Exited with error code: 3
>
> During the time I wasn't using it I think I updated Java but that was
> about it. The same files worked on another computer running XP and
> Mauve 2.3.1.
>
> At a lose and NOT computer savvy.
>
> Dave Boyd
>
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