On Apr 20, 7:26 pm, Nick Theodorakis <nick.theodora...@gmail.com> wrote: > On Apr 20, 4:58 am, Chris McDermott-Roe> > <cjmcdermott...@googlemail.com> wrote: > > Hi all, > > > I'm having a rather basic and embarassing problems with an Invitrogen NuPAGE > > gel. I'm attempting to run a regular bis-tris 4-12 % bis-tris pre-cast at > > 100 V using the supplied 20x MOPS SDS buffer but literally a second or two > > after commencing, all stops and an E1 message appears on my power supply. > > The only way I can actually get my proteins to go anywhere is by replacing > > the dummy gel which I've put in the tank with a second real gel. I'm v > > puzzled and any help is gratefully received. > > Is the buffer leaking out when a dummy gel is attached? Perhaps the > "real gels" form a better seal on the tank than the dummy.
<rant> Hm. Sometimes a simple power supply, instead of those high tech dingies (which claim that they do all the thinking for you) seems to do the trick. You simply adjust the voltage, set a maximum current and there you go. When the chamber wreaks havoc, you may turn the knobs and still get your results and don't loose your precious samples. -> Better check with those companies who provide power supplies for electronic labs, you'll get more value for your money. At least for people in Europe, this here is a good address: http://buerger-electronic.de/ (No affiliation, just a satisfied customer :) </rant> Wo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
