Just call invitrogen and tell them if they don't replace your gel doc your going to switch to biorad
Scott Brown PhD Candidate -----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of WS Sent: Wednesday, 21 April 2010 7:42 AM To: meth...@magpie.bio.indiana.edu Subject: Re: NUpage gel problem On Apr 20, 7:26 pm, Nick Theodorakis <nick.theodora...@gmail.com> wrote: > On Apr 20, 4:58 am, Chris McDermott-Roe> > <cjmcdermott...@googlemail.com> wrote: > > Hi all, > > > I'm having a rather basic and embarassing problems with an Invitrogen NuPAGE > > gel. I'm attempting to run a regular bis-tris 4-12 % bis-tris pre-cast at > > 100 V using the supplied 20x MOPS SDS buffer but literally a second or two > > after commencing, all stops and an E1 message appears on my power supply. > > The only way I can actually get my proteins to go anywhere is by replacing > > the dummy gel which I've put in the tank with a second real gel. I'm v > > puzzled and any help is gratefully received. > > Is the buffer leaking out when a dummy gel is attached? Perhaps the > "real gels" form a better seal on the tank than the dummy. <rant> Hm. Sometimes a simple power supply, instead of those high tech dingies (which claim that they do all the thinking for you) seems to do the trick. You simply adjust the voltage, set a maximum current and there you go. When the chamber wreaks havoc, you may turn the knobs and still get your results and don't loose your precious samples. -> Better check with those companies who provide power supplies for electronic labs, you'll get more value for your money. At least for people in Europe, this here is a good address: http://buerger-electronic.de/ (No affiliation, just a satisfied customer :) </rant> Wo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering. The information contained in this message and any annexure is confidential and intended only for the named recipient(s). If you have received this message in error please contact the sender immediately and destroy the original message. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
