Your gel running apparatus could be failing. Try another one. On 4/21/10 1:39 AM, "ZHOU Jie" <jet...@ust.hk> wrote:
Have you removed the white seal at the bottom of the gel? -----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Nick Theodorakis Sent: 2010年4月21日 1:27 To: meth...@magpie.bio.indiana.edu Subject: Re: NUpage gel problem On Apr 20, 4:58 am, Chris McDermott-Roe <cjmcdermott...@googlemail.com> wrote: > Hi all, > > I'm having a rather basic and embarassing problems with an Invitrogen NuPAGE > gel. I'm attempting to run a regular bis-tris 4-12 % bis-tris pre-cast at > 100 V using the supplied 20x MOPS SDS buffer but literally a second or two > after commencing, all stops and an E1 message appears on my power supply. > The only way I can actually get my proteins to go anywhere is by replacing > the dummy gel which I've put in the tank with a second real gel. I'm v > puzzled and any help is gratefully received. Is the buffer leaking out when a dummy gel is attached? Perhaps the "real gels" form a better seal on the tank than the dummy. Nick -- Nick Theodorakis nick_theodora...@hotmail.com contact form: http://theodorakis.net/contact.html _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
