Dear Sudheer, You probably get some sort of chemically not so well defined copper hydroxide (blueish-wihte, a little green) which precipitates and subsequently undergoes some more chemical changes toward CuO (you might end up with a black slurry). I doubt that this buffer system can go to 7.4. Glycine has pKa values of 2.34 and 9.60, CuCl2 should not buffer much at all. You could try set a 4M Glycine solution to pH 7.4 with solid NaOH (should be quite tricky) and then add CuCl2, then fill up get desored final concentrations Do you have a reference for that buffer - what do you want to do?
Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
