Do you really need that high molarity for both salts. That might explain the
precipitation. Rarely is copper chloride or glycine (max of 1 M) used at that
concentration for any buffers. Please double check your recipe. Perhaps
Copper dichloride is at 0.1 to 0.3 M. If you are trying to make a 10X or 100X
stock, trying making a more dilute stock.
Jay
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of sudheer babu
Sent: Tuesday, April 26, 2011 4:23 AM
To: [email protected]
Subject: Copper-Glycine buffer(Ph-7.4)
Hello everyone
I need to prepare copper choride(1M)-glycine(2M) buffer(ph.7.4). When I
dissolve these two and check that the starting Ph is 0.8. So I added NaoH
for get my desired ph and after reach the 2.4 the whole solution is
precipitating, it is not all in liquid form.
If anyone has any idea about this please answer me
Thank you in advance
Cheers
--
Sudheer Babu.S
ITC student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
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