sudheer babu <[email protected]> wrote:

>Hello everyone
>I need to prepare copper choride(1M)-glycine(2M) buffer(ph.7.4). When I
>dissolve these two and check that the starting Ph is 0.8. So I added NaoH
>for get my desired ph and after reach the 2.4 the whole solution is
>precipitating, it is not all in liquid form.

No wonder. You are precipitating Cu(OH)_2. Buffers which contain
copper and have to be basic usually contain chelating agents to keep
the copper in solution.

Christian

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