I like this discussion. A lot of info about old forgotten physical chem :) Thank you DK, Engelbert, Jayakumar, and others.
On 9 January 2012 03:08, DK <[email protected]> wrote: > In article <[email protected]>, Dr Engelbert > Buxbaum <[email protected]> wrote: > > > >Btw, if one wants to work with intracellular enzymes, PBS should not be > >used, and replaced with a high K, low Na medium! > > Well, if we are that strict then chloride salts should not be used either > :-) > Intracellular Cl- concentration is pretty low and most free anions are > large organics. For "pseudo-intracellular" solution, I've isethionate in > one system and glutamate in another. Some people like using tartrate. > > To be sure, it does make a difference. Halide ions bind proteins a lot. > > DK > > P.S. Recently I came across your book, Engelbert. Pretty good! > Made me envious :-) > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
