My pleasure Pow :-) Jay
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Pow Joshi Sent: Monday, January 09, 2012 3:18 PM To: methods Subject: Re: 10X PBS I like this discussion. A lot of info about old forgotten physical chem :) Thank you DK, Engelbert, Jayakumar, and others. On 9 January 2012 03:08, DK <[email protected]> wrote: > In article <[email protected]>, Dr Engelbert > Buxbaum <[email protected]> wrote: > > > >Btw, if one wants to work with intracellular enzymes, PBS should not be > >used, and replaced with a high K, low Na medium! > > Well, if we are that strict then chloride salts should not be used either > :-) > Intracellular Cl- concentration is pretty low and most free anions are > large organics. For "pseudo-intracellular" solution, I've isethionate in > one system and glutamate in another. Some people like using tartrate. > > To be sure, it does make a difference. Halide ions bind proteins a lot. > > DK > > P.S. Recently I came across your book, Engelbert. Pretty good! > Made me envious :-) > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
