Hi Wo, I was wondering if you could simply use the crude extract to spin down and create a sucrose density gradient...that will allow you to pick out some proteins within the gradient, and then the remaining may go over for ammonium sulphate precipitation ? ...I've no idea how this would work, but just a thought, if you have'nt already considered it.
Pow On 30 April 2012 03:52, WS <[email protected]> wrote: > Dear Experts, > > I am attempting to precipitate small amounts of (total) protein from a > fruit juice concentrate. It looks quite much like honey. My plan is to > dilute it 1x with water (to reduce viscosity) and then saturate it > with ammonium sulfate. As the solution contains high amounts of sugars > (mostly saccharose, I assume), will these interfere with my > precipitation process? > > If yes, what may I do to circumvent it? Probably dialysis, but any > other ideas? > > Thanks for your help! > > Wo > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
