What do you want to use the protein for? I had a very dilute, yet viscous extract of flowers that I wanted to use for western blotting. Carbohydrates made it visous, interfered with protein assays, and made the samples run quite poorly in a gel. I ended up using a phenol extraction method followed by MeOH precipitation that worked quite nicely. Of course, if you are trying to recover active protein this could be a problem.
Mike --- Michael L. Sullivan, PhD Research Molecular Geneticist US Dairy Forage Research Center 1925 Linden Drive Madison, WI 53706 608-890-0046 (Phone) 608-890-0076 (FAX) On Apr 30, 2012, at 2:52 AM, WS wrote: > Dear Experts, > > I am attempting to precipitate small amounts of (total) protein from a > fruit juice concentrate. It looks quite much like honey. My plan is to > dilute it 1x with water (to reduce viscosity) and then saturate it > with ammonium sulfate. As the solution contains high amounts of sugars > (mostly saccharose, I assume), will these interfere with my > precipitation process? > > If yes, what may I do to circumvent it? Probably dialysis, but any > other ideas? > > Thanks for your help! > > Wo > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
