and here's one more, from sugarcane :) http://onlinelibrary.wiley.com/doi/10.1002/elps.200900779/pdf on quick view it seems to me that they follow the method that Michael suggested, however, they also seem to have comparative yields from different methods ... hope that helps Pow
On 1 May 2012 11:52, Pow Joshi <[email protected]> wrote: > well, here's a plant phloem/Xylem sap protein isolation I found.... > http://www.springerlink.com/content/h8622j4747246137/#section=82175&page=4&locus=23 > > They use Centricon ultra filtration step to remove the sugars it seems to > me .... followed by TCA/acetone precipitation .... > > > On 1 May 2012 07:18, WS <[email protected]> wrote: > >> Hi Michael, I do not need active protein, I need it for a western. I'll >> give it a try, thanks! >> >> Wo >> >> _______________________________________________ >> Methods mailing list >> [email protected] >> http://www.bio.net/biomail/listinfo/methods >> > > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
