Hi Tomas:
My MZmine steps as following
Raw data imput
Filtering- Scan by scan filtering- Resampling filter-m/zbin length 2ppm
Peak detection- mass detection- centroid= noise 1.0 E4, MS level 1
Chromatogram builder-mass list 1= min time span 0.1, min height 1.0E4,
mass tolerance- 3ppm
P/S i had sent you the MS RAW data at
https://www.yousendit.com/download/M3BrbGtKbWdVVG1KUmNUQw.
This data was collected under Parent ion Scan. Please take look.
Thank you.
Tomas Pluskal <plus...@oist.jp> 於 2012年4月18日下午3:05 寫道：
> Hi,
>
> > Me again. Well I had tried a lot of things, including using different
> computer to run the Baseline Correction but it doesn't seems to work at
> all. I also don't know whats wrong with it.
> > Anyway i also encounter another problem regarding the Peak lists. Since
> i collect these data from the Thermo instrument i wonder MZmine 2 cope with
> Thermo data?
>
> I don't know why this happens, either.
>
> > Following is my steps by steps.
> > 1. I input my RAW data.
> > 2. Then these data underwent scan by scan filtering - Resampling filter
> (set the m/z bin at 0.5). This will generate a new raw data file named .RAW
> filtered
> > 3. Newly generated .RAW filtered file was selected for Peak Detection -
> where Centroid with 5.0E3 noise level. From here Peak list .RAW filtered
> chromatograms will be generated.
> > However, i double clicked this file and surprisingly found that there
> were nothing on the list. (the attached pic).
>
>
> Please
> 1) Send us ALL the parameter values you used for each step
> 2) Send us your data file so we can reproduce the problem
>
> > I wonder is it related to my computer problem or the incompatibility of
> MZmine with Thermo RAW files.
>
> MZmine works fine with Thermo RAW files.
>
> Tomas
>
>
> ===============================================
> Tomas Pluskal
> G0 Cell Unit, Okinawa Institute of Science and Technology Graduate
> University
> 1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan
> TEL: +81-98-966-8684
>
>
--
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