Hi Federico,

> 
> DearMzmine developers
> 
> My name is Federico Padilla, and I am a masters’ student at university of Sao 
> Paulo, Brazil. Right now in our research team (AsterBioChem - 
> http://www.asterbiochem.org/) we are working in the field of Metabolomics 
> (using a UHPLC-UV-MS Orbitrap, Exactive Plus Thermo Fisher) and the software 
> Mzmine 2.10, which we find an amazing tool, essential in any metabolomic 
> research.   
> 
> 
> 
> I am writing to you, because we are having a problem (described below) 
> treating large amounts of data in your software, using the following 
> parameters:
> 
> 1.       Mzmine version 64-bit
> 

There is no such thing as 64-bit version of MZmine...

> 2.       Java version 7 update 45
> 
Did you install 32-bit or 64-bit Java? 
What is your OS?


> 3.   
> 
>       I changed the Heap size =%total_memory% from 1024 to 512MB in order to 
> increase the JVM memory
> 
> 
>          ADDRESS_WIDTH=%%x if %ADDRESS_WIDTH%==32 (set HEAP_SIZE=4096)


This line as you changed it does not really make sense, because it means "if 
you are running a 32-bit system, set the heap size to 4GB". That can never work.


> I am analyzing about 150 plant extracts that after being transformed into 
> mzXML format add up to 10 GB of information, and when I get to the step of 
> Chromatogram builder the software crash, may be because the JVM memory gets 
> full (I am working on a computer with 8G of memory RAM and a 4 cores 
> processing unit of 2,3GHz ) based on that I tried to increase the JVM memory 
> by editing the opening link of Mzmine (it had a JVM memory of 4G and I 
> increased to 10G). However, the system is still not working. I would like to 
> know what else can I do to be able to process that information? Is there any 
> way I can do it in my computer?

Please define exactly what you mean by "crash". 

Best regards,

Tomas


===============================================
Tomas Pluskal
G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University
1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan
WWW: https://groups.oist.jp/g0
TEL: +81-98-966-8684
Fax: +81-98-966-2890

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