On Mon, Jun 29, 2009 at 12:13:46PM +0900, Namshin Kim wrote:
-> I will make AnnotationDB later, but for the variation analysis, in order to
-> save sequence information, I prefer pairwise NLMSA only for the nucleotides
-> that has been changed during sequencing. It would be better to have only one
-> NLMSA, but I rather choose to have two NLMSA (AnnotationDB & pairwise NLMSA)
-> for now. AnnotationDB for coverage and gene analysis and pairwise NLMSA for
-> variation analysis.
-> Do you think it is a good idea? I agree that it is kind of inelegant to have
-> two NLMSA... But I was afraid that the input files will be very big...

I think that's how I'm going to be doing it myself (one NLMSA for
alignment, others for annotations).  You might be interested to know
that a student is working on an NLMSA-like interface specifically for
short-read alignments, too; we should have a prototype within a few
weeks.  The goal is to have an NLMSA-compatible interface for the basic
storage.  (It will likely come to a more immediately useful resting point
than screed, which is not being worked on much over the summer while
Alex is gone.)

cheers,
--t

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