Hi James and Ali,

Are you using PyMOL 2.3 yet?

"As of PyMOL version 2.3, cartoon transparency is now an atom-level setting."

https://pymolwiki.org/index.php/Cartoon_transparency

Cheers,
  Thomas

> On Jun 26, 2019, at 10:26 AM, Ali Kusay <akus8...@uni.sydney.edu.au> wrote:
> 
> Hi James,
> 
> I am not sure what the exact cause of your issue is but I think if you make a 
> selection first then apply the transparency to the selection that should 
> work, i.e.:
> 
> sele ss H+S
> set cartoon_transparency, 0.5, sele
> 
> Something to do with selection based transparency settings not enabling newer 
> ones to be made
> 
> Cheers,
> 
> Ali
> 
> On 26/6/19, 6:18 pm, "James Starlight" <jmsstarli...@gmail.com> wrote:
> 
>    Dear pymol users!
> 
>    I am trying to set transparency based on the secondary structure using
>    pymol selection algebra
> 
>    # set transparency to all helix and sheets but not loops
>    set cartoon_transparency, 0.5, ss H+S
> 
>    which produce the following output
>    PyMOL>set cartoon_transparency, 0.5, ss H+S
>     Setting: cartoon_transparency set for 144 atoms in object 
> "0190_before_minim".
> 
>    but actually do nothing in terms of visualization.
> 
>    Could you suggest me how it would be possible to fix it?
> 
>    Thanks in advance!
> 
> 
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--
Thomas Holder
PyMOL Principal Developer
Schrödinger, Inc.



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