I really like Karrigell ( http://karrigell.sourceforge.net ).
It is, IMHO, the most pythonic framework because all you need to know
is the python language.
You don't need to learn any template or special language, you only use
plain and regular python.
It also gives you a lot of freedom when choosing a programming style:
you can code python inside html (just like in PHP or ASP) or you can
code html within python.
It also lets you map databases to objects and you can use the included
database Gadfly or any other that has a python api.
The downside: it currectly works with its built-in server, and although
you can use it alongside Apache or Xitami, there's still no way to do
it with mod_python, and as far as I know, there's no hosting providers
with Karrigell instaled.
It is being used mainly by people who run their websites from their own