Hi Uwe,

read.GanBank has problems reading scaffolds. You may use a modified version on GitHub:

https://github.com/emmanuelparadis/read.GenBank.FASTA

You can source it directly into R:

source("https://raw.githubusercontent.com/emmanuelparadis/read.GenBank.FASTA/master/read.GenBank.FASTA.R";

Best,

Emmanuel

Le 10/09/2015 16:49, Uwe Menzel a écrit :
Emmanuel Paradis <Emmanuel.Paradis@...> writes:


Hi Matt,

This is because this accession number does not point to the sequence.
For this particular one, you could use:

seq1 <- read.GenBank("U00096.3")

Best,

Emmanuel

Le 27/02/2014 01:35, Matt Curcio a écrit :
Greetings all,,
I received this error while using R version 2.15.1 and ape 3.0.11.
Error in FI[i]:LA[i] : NA/NaN argument

I have tried several approaches:

ref = "NC_000913.3"
seq1 = read.GenBank(ref)
Error in FI[i]:LA[i] : NA/NaN argument

ref = read.csv(file="test2.csv", head=T)

ref = str(ref)

# Where test2.csv is in two rows

'data.frame':   1 obs. of  1 variable:
$ seq: Factor w/ 1 level "NC+AF8-000913": 1

seq2 = read.GenBank(ref)
Error in FI[i]:LA[i] : NA/NaN argument


I was wondering if someone could lend a hand based on their own experience.






Hi,

I do have similar problems.
I try downloading 1814 sequences from K. flaccidum.

I just try with two of them:

accessions = c("DF238762", "DF238763")
flaccidum_scaffolds <- read.GenBank(accessions)

# Error in FI[i]:LA[i] : NA/NaN argument

If I try out these accesions directly in the Browser at
http://www.ncbi.nlm.nih.gov/genbank/ ,
it works very well.

So, how do I infer the "correct" accessions to be used in read.GenBank?
Or, how do I come from "NC_000913.3" to "U00096.3" in the example above?
That doesn't seem straightforward ....

Thanks, Uwe









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