I can't vouch for all-day rides I don't do, but I agree with the others that a good bike with the saddle and bars in the right places is the key to painless riding. In general I've found that padding made me less comfortable on the bicycle; even Riv's formerly-padded Summer Gloves made me feel like I was trying to push my hand through this big THING in my palm to get to the bars. I've been much more comfortable since I switched over completely to Brooks saddles, wool underwear with Riv shorts, and unpadded gloves. I used to think Spencer-at-Riv was crazy to ride that beausaged Rivendell Road without bar tape, but I'm starting to see his point.
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