Re: [spctools-discuss] Re: A question about pepXML files.

2014-02-13 Thread 钟传奇
Hannes, thanks for your reply.

You are right. ConsensusID can process the data at peptide level, and I
have done that successfully.  However, consensusID lacks validation tool at
protein level. According to a JPR paper, idXML can be converted to pepXML,
which was subsequently subjected to TPP_proteinprophet analysis.
I can not figure out how to use TPP_proteinprophet tool in openMS to
analyze pepXML, so I just ask for help here.

So the question is how to validate results at protein level in openMS.

Chuan-Qi


On Thu, Feb 13, 2014 at 9:40 PM, Hannes Röst  wrote:

> The main error seems to be that the processing engine is not recognized
>
> running: "/usr/local/apps/tpp/bin/PeptideProphetParser 'interact.pep.xml'"
>  (OpenMS/ConsensusID)
> error: engine OpenMS/ConsensusID not recognized
>
> which seems to result from the attribute
> search_engine="OpenMS/ConsensusID"
>
> however the file does contain peptideprophet_result tags so it seems
> that you may have processed them with the OpenMS  ConsensusID tool
> which does something similar to peptideprophet
>
> http://www-bs2.informatik.uni-tuebingen.de/services/OpenMS/OpenMS-release/html/TOPP_ConsensusID.html
> - is this the case?
>
> Hannes
>
> On 13 February 2014 12:08, 钟传奇  wrote:
> > The zipped pepXML file is in the attachment.
> >
> > Chuan-Qi Zhong
> >
> >
> > On Thu, Feb 13, 2014 at 7:05 PM, 钟传奇  wrote:
> >>
> >> I used openMS to process my data, and the results, idXML files, are then
> >> converted to pepXML using IDconverter in openMS.
> >> But these pepXML files cannot be processed by peptideprophet, and I
> >> noticed that peptideprophet can handle pep.XML files, so there is any
> >> difference between pepXML and pep.XML?
> >> I have attached a pepXML file.
> >>
> >> How to fix this issue?
> >>
> >> Thanks a lot.
> >>
> >> Best,
> >>
> >>
> >> Chuan-Qi Zhong
> >
> >
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Re: [spctools-discuss] Re: A question about pepXML files.

2014-02-13 Thread Hannes Röst
The main error seems to be that the processing engine is not recognized

running: "/usr/local/apps/tpp/bin/PeptideProphetParser 'interact.pep.xml'"
 (OpenMS/ConsensusID)
error: engine OpenMS/ConsensusID not recognized

which seems to result from the attribute
search_engine="OpenMS/ConsensusID"

however the file does contain peptideprophet_result tags so it seems
that you may have processed them with the OpenMS  ConsensusID tool
which does something similar to peptideprophet
http://www-bs2.informatik.uni-tuebingen.de/services/OpenMS/OpenMS-release/html/TOPP_ConsensusID.html
- is this the case?

Hannes

On 13 February 2014 12:08, 钟传奇  wrote:
> The zipped pepXML file is in the attachment.
>
> Chuan-Qi Zhong
>
>
> On Thu, Feb 13, 2014 at 7:05 PM, 钟传奇  wrote:
>>
>> I used openMS to process my data, and the results, idXML files, are then
>> converted to pepXML using IDconverter in openMS.
>> But these pepXML files cannot be processed by peptideprophet, and I
>> noticed that peptideprophet can handle pep.XML files, so there is any
>> difference between pepXML and pep.XML?
>> I have attached a pepXML file.
>>
>> How to fix this issue?
>>
>> Thanks a lot.
>>
>> Best,
>>
>>
>> Chuan-Qi Zhong
>
>
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