Hi all,
Great idea! As being relatively new to the field, I definitely find
this idea appealing. Actually hearing the talks would indeed be quite
educational.
Cheers,
Ronnie Berntsson
On Aug 23, 2007, at 10:57 AM, Clemens Vonrhein wrote:
Hi Eleanore,
On Tue, Aug 21, 2007 at 12:56:40PM
Dear group members,
I would like to bring following the openings to your attention:
ANNOUNCEMENT TITLE: Postdoctoral positions in crystallography in
Stockholm, Sweden
DEADLINE FOR APPLICATIONS: 25 October 2007
TYPE OF POSITION: Post-Doc(s)
INSTITUTION/DEPARTMENT: Center for Infectious Medicine,
Mischa,
I don't think that the field of nanotechnology crumbled when allegations
against Jan Hendrik Schon (21 papers withdrawn, 15 in Science/Nature)
turned out to be true. I don't think that nobody trusts biologists
anymore because of Eric Poehlman (17 falsified grants, 10 papers with
Dear colleagues,
1) I think Ajees et al. should make available the raw diffraction images of
the structure in paper that has caused so much literary commotion, unless
they haven't already done so. Perhaps simply put them in an open ftp server?
As I imagine, unless I have missed something, these
Hi Shivesh
I had something like this recently and found that changing the pH stopped
the precipitation and the size of the crystals improved. If the crystals
are large enough even if there is ppt present I would still try and
collect data on them. This is likely not the case for you or you
Sorry for the repost, but I think my question got lost in the earlier
thread.
I've found
$CCP4/x-windows/ipdisp/src/pack_c.c, pack_f.f and so forth, but they
apparently don't build by default, and when I try to, I get
You need to make mosflm-bits in the library for the image-packing stuff
Well, I know it's not the definitive source of anything, but the
wikipedia entry on JPEG2000 says:
The PNG (Portable Network Graphics) format is still more
space-efficient in the case of images with many pixels of the same
color, and supports special compression features that JPEG 2000 does
Hi Bill,
It seems it would be good to have available if we are all going to
put our images on our web-servers.
Probably easier to either just bzip2 the images (which works reasonably well
but is somewhat slow) or use one of the imgCIF jiffy programs to do this,
which will correctly retain
Sorry - this may have been mentioned previously, but have you tried banging in
some glycerol (5-10%)?
J
shivesh kumar [EMAIL PROTECTED] wrote:
Dear all
I welcome all the suggestions regarding my crystals which is coming with
the
precipitation.The pI of the protein is 4.2 and the drop
I agree to this last suggestion. For one of my protein, I had to add at
least 10 % of glycerol in my buffers to keep my protein stable while
purifying. For crystallization, I diluted it to 20 % glycerol (vapour
diffusion method) which allowed it to be stable in the crystallization
drop and
Is there any advantage to bzip2-ing the individual images rather than
making one bzip2-ed tarball with tar cvfj?
Yes. If you have folks sending you images by sftp or e-mail, then you
don't have ensure a tarball of Giga or Tera bytes works. You can send the
smaller multiple files and restart
Hello All,
I have a question about some data with which I have been grappling. The crystal
appears to be primitive cubic(p23). Are there alternate ways to index cubic
data? I ask this question without regard to anomalous data. I have looked at
several datasets in Xtriage. I wanted to merge
I've been reading the contributions on this topic with much interest.
It's been very timely in that I've been giving 3rd year u/g lectures
on protein X-ray structures and their validation over the past week.
As part of the preparation for the lectures, I searched the PDB for
structures with
Hi all,
Great idea! As being reasonably new to the field, I would definitely
welcome those videos. Not that I don't read Acta D papers, but it is
always nice to hear the talks directly, and would certainly be quite
educational.
Cheers,
Ronnie Berntsson
--
Ph.D. Student
In the cases you list, it is clearly recognized that the fault lies
with the investigator and not the method. In most of the cases where
serious problems have been identified in published models the authors
have stonewalled by saying that the method failed them.
The methods of
- Original Message -
From: Jenny Martin [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, August 23, 2007 5:46 PM
Subject: Re: [ccp4bb] The importance of USING our validation tools
My question is, how could crystals with 80% or more solvent diffract so
well? The best of the
Another example of a structure with intervening
layers of weak electron density at 1.75 A
resolution is Pb2+ bound calmodulin that Mark Wilson
solved in my laboratory: M.A. Wilson and A.T. Brunger, /
Acta Cryst./D59, 1782-1792 (2003), PDB ID 1NOY.
The intervening layers are not entirely
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