Roger,
Very low values of Kd (nM) may mean that you have a good chance of finding both
the proteins (or protein-nucleic acid) together if you get crystals of the
mixture. I therefore think that the measurements are useful in that sense.
However, low Kd does not necessarily mean that you get th
Tim,
My understanding is that the D-isomer does not get incorporated. I could be
wrong though. Bacteria may convert the D to the L-form.
Chitta
- Original Message -
From: "Tim Gruene"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, November 12, 2012 4:25:34 AM
Subject: Re: [ccp4bb] seleno
Dee,
Intramolecular electrostatic interactions are common in protein structures, for
example between i and i+4 pairs on the same face of a helix. Robert Baldwin
(Stanford) and others did a lot of work on the contribution of intramolecular
salt bridges to helix stabilization in model helices. If
Veerendra,
You can rule out if zinc has replaced calcium ions (although I agree with Nat
and others that looking at the coordination sphere should give a big clue) by
taking a few crystals, washing them a couple of times and subjecting them to
ICP-MS analysis, if you have access to this techni
Peter,
I think it would depend if the substrate analog have ionizable groups? If the
analog does not have ionizable groups, it is hard to imagine how the the
titration of ionizable groups on the protein would impair the binding.
Chitta
- Original Message -
From: "Peter Hsu"
To: CC
I agree with Garib that we should stop this, because nothing productive seems
to be coming out of this discussion.
I however like to clarify one thing about the comment I made about agism. I
merely intended to interpret what the age limit preference means in the context
of Government of India's
Please allow me to share with you some history here. Late Prof. GN Ramachandran
(of Ramachandran Plot) was a faculty of MBU, IISC. If you want to find out
yourself how good/bad it is, please register for the upcoming conference on
"International Conference on Biomolecular Forms and Functions: c